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Metal binding to D-lactate dehydrogenase
Biochemistry, 1982The kinetic and spectral properties of native and cobalt-substituted D-lactate dehydrogenase have been compared. Optical and MCD spectra are consistent with high-spin tetrahedral cobalt. The cobalt enzyme is strongly inhibited by D-lactate when oxygen is the electron acceptor.
F F, Morpeth, V, Massey
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D(—)-Lactate Dehydrogenase in Lower Fungi
Science, 1966A lactate dehydrogenase, specific for nicotinamide-adenine dinucleotide and D(—)-lactate, has been detected in extracts from two fungi of the order Leptomitales (Oomycetes). Several fungi of this order carry out a lactic acid fermentation under conditions of reduced oxygen tension.
Frank H. Gleason +3 more
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Colorimetric measurement of D(-)lactate in plasma
Clinical Biochemistry, 1987We describe a method for the direct assay of D(-)lactate in plasma using D(-)lactate dehydrogenase and a color reagent to produce a formazan color measured at 510 nm. This method agrees well with a modified lactate UV end-point method r = 0.998 and the regression equation y = 0.974x + 0.13.
J E, Buttery, P R, Pannall
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D-Lactate metabolism in starvedOctopus ocellatus
Journal of Experimental Zoology Part A: Comparative Experimental Biology, 2005The concentrations of D- and L-lactate, methylglyoxal and pyruvate were measured in tissues of normal and starved Octopus ocellatus. D-Lactate was always more abundant than L-lactate in the tissues. D-Lactate, pyruvate and methylglyoxal were present in 320, 94 and 43 times higher concentrations in tentacle of O. ocellatus of control group than those in
Tomomi, Fujisawa +4 more
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METABOLISM OF SODIUM d-LACTATE
Archives of Internal Medicine, 1937The metabolism of racemic sodium lactate in the presence of disease of the liver has been studied by Schumacher 1 and Hartmann and Senn. 2 The two optically active enantiomer components constituting racemic lactate differ from each other, according to Cori and Cori, 3 with regard to the manner of their biologic utilization.
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Production of d(−)-lactate from sucrose and molasses
Biotechnology Letters, 2004Escherichia coli W3110 derivatives, strains SZ63 and SZ85, were previously engineered to produce optically pure D(-) and L(+)-lactate from hexose and pentose sugars. To expand the substrate range, a cluster of sucrose genes (cscR' cscA cscKB) was cloned and characterized from E. coli KO11.
V B, Shukla +5 more
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Metabolism of D-Lactate in the Dog and in Man
Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis, 1989Peritoneal fluid contains equimolar amounts of D and L isomers of lactate. Controversy exists as to whether D-Iactate is metabolized as effectively as the L isomer. D and L-Iactate were infused into the portal circulation of dogs and net hepatic uptake of both isomers measured by A-V sampling, blood flows being measured by flow probes.
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Journal of Pharmaceutical and Biomedical Analysis, 2015
In this study, we aimed to develop a new enzymatic assay system of d-lactate with good precision, accuracy, and sensitivity for the determination of D-lactate concentrations in rat serum. D-Lactate dehydrogenase (D-LDH) was utilized to catalyze D-lactate and NAD(+) to pyruvate and NADH, respectively.
Chien-Ming, Chen +3 more
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In this study, we aimed to develop a new enzymatic assay system of d-lactate with good precision, accuracy, and sensitivity for the determination of D-lactate concentrations in rat serum. D-Lactate dehydrogenase (D-LDH) was utilized to catalyze D-lactate and NAD(+) to pyruvate and NADH, respectively.
Chien-Ming, Chen +3 more
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D‐lactate‐induced neurotoxicity in calves
Journal of Animal Physiology and Animal Nutrition, 2008D‐lactic acidosis occurs as a complication of short‐bowel syndrome in humans and in a variety of other gastrointestinal disorders in monogastrics and ruminants. It is associated with signs of impaired central nervous system (CNS) function including ataxia and coma.
J. M. Naylor +4 more
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Fluorometric determination of d-lactate in biological fluids
Analytical Biochemistry, 2017D-lactic acid in the mammalian body is mainly of microbiological origin and is often located somewhere along the digestive tract. Surgical, extensive re-sectioning of the small bowel may be one of the risk factors for altered balance in the microbiological environment.
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