Results 151 to 160 of about 241,206 (292)
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
The mixture experiments (MAE) were influenced by both the proportions of the components and the total amount. The traditional MAE encompasses classical mixture experiments for each total amount, which complicates the application of complete randomization;
Angriany, A.Muthiah Nur +1 more
core +1 more source
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
Exact D-optimal designs for mixture experiments in Scheffe's quadratic models
, 2006 The exact D-optimal design problems for regression models has been in-vestigated in many literatures. Huang (1987) and Gaffke (1987) provided a sufficient condition for the minimum sample size for an certain set of candidate designs to be exact D-optimal
Wu, Shian-Chung
core
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
D-optimal design for Becker's minimum polynomial
Becker's minimum polynomial (e.g. Becker, 1968, J. Roy. Statist. Soc. Ser. B 30, 349-358.) of order [nu] on the (unit) q-simplex including the minimum functions over all subsets of at most [nu][less-than-or-equals, slant]q variables is considered.
Hilgers, Ralf-Dieter
core
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
International Journal of Nanomedicine, 2019 Nadiatul Atiqah Wahgiman1, Norazlinaliza Salim1,2, Mohd Basyaruddin Abdul Rahman1, Siti Efliza Ashari1,2 1Integrated Chemical BioPhysics Research, Faculty of Science, University Putra Malaysia (UPM), Serdang, Selangor 43400, Malaysia; 2Centre of ...
Wahgiman NA +3 more
doaj
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source