Results 101 to 110 of about 849 (141)

Characterization of a d-psicose-producing enzyme, d-psicose 3-epimerase, from Clostridium sp.

Biotechnology Letters, 2013
The gene coding for D-psicose 3-epimerase (DPEase) from Clostridium sp. BNL1100 was cloned and expressed in Escherichia coli. The recombinant enzyme was purified by Ni-affinity chromatography. It was a metal-dependent enzyme and required Co(2+) as optimum cofactor.
Wanmeng Mu, Bo Jiang, Wenli Zhang
exaly   +3 more sources

Improved operational stability of d -psicose 3-epimerase by a novel protein engineering strategy, and d -psicose production from fruit and vegetable residues

Bioresource Technology, 2016
The aim of the present work was to improve stability of d-psicose 3-epimerase and biotransformation of fruit and vegetable residues for d-psicose production. The study established that N-terminal fusion of a yeast homolog of SUMO protein - Smt3 - can confer elevated optimal temperature and improved operational stability to d-psicose 3-epimerase.
Sudhir P Singh, Satya Narayan Patel
exaly   +3 more sources

Recent advances on applications and biotechnological production of d-psicose

Applied Microbiology and Biotechnology, 2012
D-Psicose is a hexoketose monosaccharide sweetener, which is a C-3 epimer of D-fructose and is rarely found in nature. It has 70 % relative sweetness but 0.3 % energy of sucrose, and is suggested as an ideal sucrose substitute for food products. It shows important physiological functions, such as blood glucose suppressive effect, reactive oxygen ...
Wanmeng Mu, Bo Jiang, Wenli Zhang
exaly   +3 more sources

Production of d-psicose from d-fructose by whole recombinant cells with high-level expression of d-psicose 3-epimerase from Agrobacterium tumefaciens

Journal of Bioscience and Bioengineering, 2016
The specific activity of recombinant Escherichia coli cells expressing the double-site variant (I33L-S213C) d-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens was highest at 24 h of cultivation time in Terrific Broth (TB) medium among the media tested.
Chang-Su Park, Kyung-Chul Shin, Deok-Kun Oh   +2 more
exaly   +3 more sources

D‐psicose 3‐epimerase secretory overexpression, immobilization, and d‐psicose biotransformation, separation and crystallization

Journal of Chemical Technology & Biotechnology, 2017
AbstractBACKGROUNDD‐psicose is a rare sugar and exists in extremely small quantities in nature. It has important physiological functions and is allowed to be used as an ingredient in foods and dietary supplements. The aim of this study is to develop the biotransformation, separation and purification methods for highly efficient mass production of d ...
Can Li, Jianqun Lin, Qingqing Guo, Chengjia Zhang, Kai Du, Huibin Lin, Jianqiang Lin   +6 more
openaire   +1 more source

Synthesis and stereochemistry of some derivatives of d-psicose

Carbohydrate Research, 1979
Abstract A number of derivatives of the ketohexose d -psicose have been synthesized, comprising examples of acetals, esters, ethers, and glycosides. Among them are precursors of 1-,3-,5-, and 6-mono- O -methyl- d -psicose, including 3- O -methyl-β- d -psicopyranose 4,5-carbonate, the first crystalline representative of the reducing form of the ...
Philippe C.M. Herve Du Penhoat, Arthur S. Perlin   +1 more
openaire   +1 more source

A d-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for d-psicose production: cloning, expression, purification, and characterization

Applied Microbiology and Biotechnology, 2013
D-Tagatose 3-epimerase family enzymes can efficiently catalyze the epimerization of free keto-sugars, which could be used for D-psicose production from D-fructose. In previous studies, all optimum pH values of these enzymes were found to be alkaline. In this study, a D-psicose 3-epimerase (DPEase) with neutral pH optimum from Clostridium bolteae (ATCC ...
Min, Jia, Wanmeng, Mu, Feifei, Chu, Xiaoming, Zhang, Bo, Jiang, Liuming Leon, Zhou, Tao, Zhang   +6 more
openaire   +2 more sources

d-Allose and d-psicose reinforce the action of metronidazole on trichomonad

Parasitology Research, 2011
The effects of D-allose and D-psicose on Tritrichomonas foetus were examined. They were cultured in F-bouillon medium including glucose, but had never increased when glucose was substituted to those sugars. When cultured in a medium including a dose of ED(50) metronidazole and those sugars, trichomonad density was significantly less than that in a ...
Masakazu, Harada, Emi, Kondo, Hiromi, Hayashi, Chigusa, Suezawa, Setsuo, Suguri, Meiji, Arai   +5 more
openaire   +2 more sources

Efficient conversion of allitol to d-psicose by Bacillus pallidus Y25

Journal of Bioscience and Bioengineering, 2007
An efficient method for conversion of allitol to D-psicose was achieved by a resting cell reaction of Bacillus pallidus Y25 for the first time. Notably, it was possible to produce D-allose and D-altrose from allitol directly via D-psicose by prolonging the reaction time. This method was applied for the preparation of D-psicose using the extract of Itea
Wayoon, Poonperm, Goro, Takata, Yasuyo, Ando, Verasak, Sahachaisaree, Pipob, Lumyong, Saisamorn, Lumyong, Ken, Izumori   +6 more
openaire   +2 more sources

Chitosan Productivity Enhancement in Rhizopus oryzae YPF-61A by D-Psicose

Journal of Bioscience and Bioengineering, 2003
The effect of the rare sugar D-psicose on chitosan production by Rhizopus oryzae was studied. The fungus was not able to utilize D-psicose as a sole source of carbon, either for germination of the spores or for growth of the vegetative cells. In a medium containing a low amount of D-glucose, however, D-psicose supplementation between 5 and 12 g/l ...
Kazutoshi, Yoshihara, Yoshihiro, Shinohara, Takahiro, Hirotsu, Ken, Izumori   +3 more
openaire   +2 more sources