Results 101 to 110 of about 29,402,319 (134)

Efficient biosynthesis of D-allulose in Bacillus subtilis through D-psicose 3-epimerase translation modification

International Journal of Biological Macromolecules, 2021
The combined catalysis of glucose isomerase (GI) and D-psicose 3-epimerase (DPEase) provided a convenient route for the direct synthesis of D-allulose from d-glucose, whose cost is lower than d-fructose. In the present research, the weak activity of DPEase was the key rate-limiting step and resulted in the accumulation of d-fructose in engineered ...
Jingyi, Zhao, Hongbei, Wei, Jing, Chen, Lihong, Li, Kai, Li, Jidong, Liu   +5 more
openaire   +2 more sources

Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose

Enzyme and Microbial Technology, 2014
D-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of D-psicose production, thermoactive D-glucose isomerase and the D-psicose 3-epimerase obtained from Bacillus sp.
Yan, Men, Yueming, Zhu, Yan, Zeng, Ken, Izumori, Yuanxia, Sun, Yanhe, Ma   +5 more
openaire   +2 more sources

Chaperone-mediated protein folding enhanced D-psicose 3-epimerase expression in engineered Bacillus subtilis

Process Biochemistry, 2021
Abstract Functional mature proteins usually undergo folding, and in vivo folding is often affected by factors such as translation ratio, intracellular environment, and chaperones. d -psicose 3-epimerase (DPEase) derived from Dorea sp. has excellent properties in catalytic production of d -allulose; a rare sugar with unique biological functions ...
Jing Chen, Hongbei Wei, Yan Guo, Qiufeng Li, Huiyi Wang, Jidong Liu   +5 more
openaire   +1 more source

Deactivation kinetics and the effects of additives on storage stability and structure of d-psicose 3-epimerase

Biotechnology Letters, 2017
To explore deactivation kinetics and the effects of some additives on the activity and conformational changes of D-psicose 3-epimerase (DPEase) during its storage.The experimental data of DPEase inactivation during storage at 4-45 °C fitted with the first-order expression model.
Qiao, Zhang, Bo, Jiang, Tao, Zhang, Zhenhua, Duan   +3 more
openaire   +2 more sources

Improved operational stability of d -psicose 3-epimerase by a novel protein engineering strategy, and d -psicose production from fruit and vegetable residues

Bioresource Technology, 2016
The aim of the present work was to improve stability of d-psicose 3-epimerase and biotransformation of fruit and vegetable residues for d-psicose production. The study established that N-terminal fusion of a yeast homolog of SUMO protein - Smt3 - can confer elevated optimal temperature and improved operational stability to d-psicose 3-epimerase.
Satya Narayan, Patel, Manisha, Sharma, Kusum, Lata, Umesh, Singh, Vinod, Kumar, Rajender S, Sangwan, Sudhir P, Singh   +6 more
openaire   +2 more sources

Production of d-psicose from d-fructose by whole recombinant cells with high-level expression of d-psicose 3-epimerase from Agrobacterium tumefaciens

Journal of Bioscience and Bioengineering, 2016
The specific activity of recombinant Escherichia coli cells expressing the double-site variant (I33L-S213C) d-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens was highest at 24 h of cultivation time in Terrific Broth (TB) medium among the media tested.
Chang-Su, Park, Chul-Soon, Park, Kyung-Chul, Shin, Deok-Kun, Oh   +3 more
openaire   +2 more sources

Preparation of d-psicose from d-fructose by immobilized d-tagatose 3-epimerase

Journal of Fermentation and Bioengineering, 1995
Abstract d -Tagatose 3-epimerase ( d -TE) from Pseudomonas sp. ST-24 immobilized on Chitopearl beads of BCW 2503 was used in the preparation of d -psicose from d -fructose. When d -fructose solution (10%) was passed through a column packed with immobilized d -TE about 20% of the d -fructose was converted to d -psicose.
Hiromichi Itoh, Tomoko Sato, Ken Izumori
openaire   +1 more source

Immobilization on graphene oxide improves the thermal stability and bioconversion efficiency of D-psicose 3-epimerase for rare sugar production

Enzyme and Microbial Technology, 2017
D-Psicose (D-ribo-2-hexulose or D-allulose), an epimer of D-fructose is considered as a rare low-calorie sugar displaying important physiological functions. Enzymatic production using ketose 3-epimerases is the feasible process for the production of D-Psicose.
Samir R, Dedania, Manisha J, Patel, Dijit M, Patel, Rekha C, Akhani, Darshan H, Patel   +4 more
openaire   +2 more sources

Production of d-allose from d-fructose using immobilized l-rhamnose isomerase and d-psicose 3-epimerase

Bioprocess and Biosystems Engineering, 2019
D-Allose is a rare sugar, can be used as an ingredient in a range of foods and dietary supplements, has alimentary activities, especially excellent anti-cancer effects and used in assisting cancer chemotherapy and radiotherapy, etc. To develop a simple and low-cost process for D-allose production, a one-pot enzymatic process using the substrate of D ...
Can, Li, Ling, Gao, Kai, Du, Huibin, Lin, Yilin, Ren, Jianqun, Lin, Jianqiang, Lin   +6 more
openaire   +2 more sources

High-level extracellular production of d-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach

Bioprocess and Biosystems Engineering, 2013
The aim of this study is to achieve high-level extracellular production of D-Psicose-3-epimerase (DPE) with recombinant Escherichia coli. High-level production of DPE is one of the key factors in D-Psicose production. In the present study, the gene AAL45544.1 from Agrobacterium tumefaciens str.
Lei, Gu, Juan, Zhang, Baihong, Liu, Chongde, Wu, Guocheng, Du, Jian, Chen   +5 more
openaire   +2 more sources