Results 111 to 120 of about 509 (137)

Improved operational stability of d -psicose 3-epimerase by a novel protein engineering strategy, and d -psicose production from fruit and vegetable residues

Bioresource Technology, 2016
The aim of the present work was to improve stability of d-psicose 3-epimerase and biotransformation of fruit and vegetable residues for d-psicose production. The study established that N-terminal fusion of a yeast homolog of SUMO protein - Smt3 - can confer elevated optimal temperature and improved operational stability to d-psicose 3-epimerase.
Satya Narayan, Patel, Manisha, Sharma, Kusum, Lata, Umesh, Singh, Vinod, Kumar, Rajender S, Sangwan, Sudhir P, Singh   +6 more
openaire   +4 more sources

A stable immobilized d-psicose 3-epimerase for the production of d-psicose in the presence of borate

Process Biochemistry, 2009
Abstract Maximal activity of the immobilized d -psicose 3-epimerase from Agrobacterium tumefaciens on Duolite A568 beads was achieved at pH 9.0 and 55 °C with borate, and at pH 8.5 and 50 °C without borate. The half-lives of the immobilized enzyme at 50 °C with and without borate were increased 4.2- and 128-fold compared to that of the free enzyme ...
Byung-Chul Lim, Hye-Jung Kim, Deok-Kun Oh   +2 more
openaire   +3 more sources

Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose

Enzyme and Microbial Technology, 2014
D-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of D-psicose production, thermoactive D-glucose isomerase and the D-psicose 3-epimerase obtained from Bacillus sp.
Yan, Men, Yueming, Zhu, Yan, Zeng, Ken, Izumori, Yuanxia, Sun, Yanhe, Ma   +5 more
openaire   +4 more sources

Characterization of a d-psicose-producing enzyme, d-psicose 3-epimerase, from Clostridium sp.

Biotechnology Letters, 2013
The gene coding for D-psicose 3-epimerase (DPEase) from Clostridium sp. BNL1100 was cloned and expressed in Escherichia coli. The recombinant enzyme was purified by Ni-affinity chromatography. It was a metal-dependent enzyme and required Co(2+) as optimum cofactor.
Wanmeng, Mu, Wenli, Zhang, Dan, Fang, Leon, Zhou, Bo, Jiang, Tao, Zhang   +5 more
openaire   +4 more sources

Overexpression of d-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in d-psicose production

Biotechnology Letters, 2012
The D-psicose 3-epimerase (DPE) gene from Ruminococcus sp. was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. It was optimally active at pH 7.5-8.0 and 60 °C. Activity was not dependent on the presence of metal ions; however, it became more thermostable with added Mn(2+).
Yueming, Zhu, Yan, Men, Wei, Bai, Xiaobo, Li, Lili, Zhang, Yuanxia, Sun, Yanhe, Ma   +6 more
openaire   +4 more sources

A d-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for d-psicose production: cloning, expression, purification, and characterization

Applied Microbiology and Biotechnology, 2013
D-Tagatose 3-epimerase family enzymes can efficiently catalyze the epimerization of free keto-sugars, which could be used for D-psicose production from D-fructose. In previous studies, all optimum pH values of these enzymes were found to be alkaline. In this study, a D-psicose 3-epimerase (DPEase) with neutral pH optimum from Clostridium bolteae (ATCC ...
Min, Jia, Wanmeng, Mu, Feifei, Chu, Xiaoming, Zhang, Bo, Jiang, Liuming Leon, Zhou, Tao, Zhang   +6 more
openaire   +4 more sources

Production of d-psicose from d-fructose by whole recombinant cells with high-level expression of d-psicose 3-epimerase from Agrobacterium tumefaciens

Journal of Bioscience and Bioengineering, 2016
The specific activity of recombinant Escherichia coli cells expressing the double-site variant (I33L-S213C) d-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens was highest at 24 h of cultivation time in Terrific Broth (TB) medium among the media tested.
Chang-Su, Park, Chul-Soon, Park, Kyung-Chul, Shin, Deok-Kun, Oh   +3 more
openaire   +4 more sources

Preparation of d-psicose from d-fructose by immobilized d-tagatose 3-epimerase

Journal of Fermentation and Bioengineering, 1995
Abstract d -Tagatose 3-epimerase ( d -TE) from Pseudomonas sp. ST-24 immobilized on Chitopearl beads of BCW 2503 was used in the preparation of d -psicose from d -fructose. When d -fructose solution (10%) was passed through a column packed with immobilized d -TE about 20% of the d -fructose was converted to d -psicose.
Hiromichi Itoh, Tomoko Sato, Ken Izumori
openaire   +3 more sources

Mass production of d-psicose from d-fructose by a continuous bioreactor system using immobilized d-tagatose 3-epimerase

Journal of Bioscience and Bioengineering, 2000
An improved process for the mass production of D-psicose from D-fructose was developed. A D-fructose solution (60%, pH 7.0) was passed at 45 degrees C through a column filled with immobilized D-tagatose 3-epimerase (D-TE) which was produced using recombinant Escherichia coli, and 25% of the substrate was converted to D-psicose. After epimerization, the
K, Takeshita, A, Suga, G, Takada, K, Izumori   +3 more
exaly   +6 more sources

Immobilization on graphene oxide improves the thermal stability and bioconversion efficiency of D-psicose 3-epimerase for rare sugar production

Enzyme and Microbial Technology, 2017
D-Psicose (D-ribo-2-hexulose or D-allulose), an epimer of D-fructose is considered as a rare low-calorie sugar displaying important physiological functions. Enzymatic production using ketose 3-epimerases is the feasible process for the production of D-Psicose.
Samir R, Dedania, Manisha J, Patel, Dijit M, Patel, Rekha C, Akhani, Darshan H, Patel   +4 more
openaire   +4 more sources