Results 111 to 120 of about 1,864,743 (129)
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International Journal of Biological Macromolecules, 2021
The combined catalysis of glucose isomerase (GI) and D-psicose 3-epimerase (DPEase) provided a convenient route for the direct synthesis of D-allulose from d-glucose, whose cost is lower than d-fructose. In the present research, the weak activity of DPEase was the key rate-limiting step and resulted in the accumulation of d-fructose in engineered ...
Jingyi, Zhao +5 more
openaire +2 more sources
The combined catalysis of glucose isomerase (GI) and D-psicose 3-epimerase (DPEase) provided a convenient route for the direct synthesis of D-allulose from d-glucose, whose cost is lower than d-fructose. In the present research, the weak activity of DPEase was the key rate-limiting step and resulted in the accumulation of d-fructose in engineered ...
Jingyi, Zhao +5 more
openaire +2 more sources
Journal of Chemical Technology & Biotechnology, 2017
AbstractBACKGROUNDD‐psicose is a rare sugar and exists in extremely small quantities in nature. It has important physiological functions and is allowed to be used as an ingredient in foods and dietary supplements. The aim of this study is to develop the biotransformation, separation and purification methods for highly efficient mass production of d ...
Can Li +6 more
openaire +1 more source
AbstractBACKGROUNDD‐psicose is a rare sugar and exists in extremely small quantities in nature. It has important physiological functions and is allowed to be used as an ingredient in foods and dietary supplements. The aim of this study is to develop the biotransformation, separation and purification methods for highly efficient mass production of d ...
Can Li +6 more
openaire +1 more source
Process Biochemistry, 2021
Abstract Functional mature proteins usually undergo folding, and in vivo folding is often affected by factors such as translation ratio, intracellular environment, and chaperones. d -psicose 3-epimerase (DPEase) derived from Dorea sp. has excellent properties in catalytic production of d -allulose; a rare sugar with unique biological functions ...
Jing Chen +5 more
openaire +1 more source
Abstract Functional mature proteins usually undergo folding, and in vivo folding is often affected by factors such as translation ratio, intracellular environment, and chaperones. d -psicose 3-epimerase (DPEase) derived from Dorea sp. has excellent properties in catalytic production of d -allulose; a rare sugar with unique biological functions ...
Jing Chen +5 more
openaire +1 more source
Journal of Bioscience and Bioengineering, 2000
An improved process for the mass production of D-psicose from D-fructose was developed. A D-fructose solution (60%, pH 7.0) was passed at 45 degrees C through a column filled with immobilized D-tagatose 3-epimerase (D-TE) which was produced using recombinant Escherichia coli, and 25% of the substrate was converted to D-psicose. After epimerization, the
Kei Takeshita
exaly +5 more sources
An improved process for the mass production of D-psicose from D-fructose was developed. A D-fructose solution (60%, pH 7.0) was passed at 45 degrees C through a column filled with immobilized D-tagatose 3-epimerase (D-TE) which was produced using recombinant Escherichia coli, and 25% of the substrate was converted to D-psicose. After epimerization, the
Kei Takeshita
exaly +5 more sources
Biotechnology Letters, 2017
To explore deactivation kinetics and the effects of some additives on the activity and conformational changes of D-psicose 3-epimerase (DPEase) during its storage.The experimental data of DPEase inactivation during storage at 4-45 °C fitted with the first-order expression model.
Qiao, Zhang +3 more
openaire +2 more sources
To explore deactivation kinetics and the effects of some additives on the activity and conformational changes of D-psicose 3-epimerase (DPEase) during its storage.The experimental data of DPEase inactivation during storage at 4-45 °C fitted with the first-order expression model.
Qiao, Zhang +3 more
openaire +2 more sources
Preparation of d-psicose from d-fructose by immobilized d-tagatose 3-epimerase
Journal of Bioscience and Bioengineering, 1995Abstract d -Tagatose 3-epimerase ( d -TE) from Pseudomonas sp. ST-24 immobilized on Chitopearl beads of BCW 2503 was used in the preparation of d -psicose from d -fructose. When d -fructose solution (10%) was passed through a column packed with immobilized d -TE about 20% of the d -fructose was converted to d -psicose.
Hiromichi Itoh, Tomoko Sato, Ken Izumori
exaly +2 more sources
Journal of Molecular Catalysis B: Enzymatic, 2015
Abstract Ketose 3-epimerase displayed an important role in not only the cyclic monosaccharides bioconversion strategy, named Izumoring, but also in the industrial biological production of d -psicose, a novel low-calorie rare sugar widely used in food and medical industries.
Wenli Zhang, Bo Jiang, Leon Zhou
exaly +2 more sources
Abstract Ketose 3-epimerase displayed an important role in not only the cyclic monosaccharides bioconversion strategy, named Izumoring, but also in the industrial biological production of d -psicose, a novel low-calorie rare sugar widely used in food and medical industries.
Wenli Zhang, Bo Jiang, Leon Zhou
exaly +2 more sources
Enzyme and Microbial Technology, 2017
D-Psicose (D-ribo-2-hexulose or D-allulose), an epimer of D-fructose is considered as a rare low-calorie sugar displaying important physiological functions. Enzymatic production using ketose 3-epimerases is the feasible process for the production of D-Psicose.
Samir R Dedania +2 more
exaly +3 more sources
D-Psicose (D-ribo-2-hexulose or D-allulose), an epimer of D-fructose is considered as a rare low-calorie sugar displaying important physiological functions. Enzymatic production using ketose 3-epimerases is the feasible process for the production of D-Psicose.
Samir R Dedania +2 more
exaly +3 more sources
Enzyme and Microbial Technology, 2020
d-Psicose (d-ribo-2-hexulose or d-allulose) is the Carbon-3 epimer of d-fructose sugar and considered as an unnatural (rare) sugar found in low amount in nature. It has about 70% of the relative sweetness but 0.3% of the energy of sucrose, which is suggested as the most suitable sucrose substitute for food additives. Enzymatic biosynthesis using ketose
Samir R Dedania +2 more
exaly +3 more sources
d-Psicose (d-ribo-2-hexulose or d-allulose) is the Carbon-3 epimer of d-fructose sugar and considered as an unnatural (rare) sugar found in low amount in nature. It has about 70% of the relative sweetness but 0.3% of the energy of sucrose, which is suggested as the most suitable sucrose substitute for food additives. Enzymatic biosynthesis using ketose
Samir R Dedania +2 more
exaly +3 more sources
Bioprocess and Biosystems Engineering, 2019
D-Allose is a rare sugar, can be used as an ingredient in a range of foods and dietary supplements, has alimentary activities, especially excellent anti-cancer effects and used in assisting cancer chemotherapy and radiotherapy, etc. To develop a simple and low-cost process for D-allose production, a one-pot enzymatic process using the substrate of D ...
Can, Li +6 more
openaire +2 more sources
D-Allose is a rare sugar, can be used as an ingredient in a range of foods and dietary supplements, has alimentary activities, especially excellent anti-cancer effects and used in assisting cancer chemotherapy and radiotherapy, etc. To develop a simple and low-cost process for D-allose production, a one-pot enzymatic process using the substrate of D ...
Can, Li +6 more
openaire +2 more sources