Results 131 to 140 of about 1,259 (144)

High-level extracellular production of d-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach

Bioprocess and Biosystems Engineering, 2013
The aim of this study is to achieve high-level extracellular production of D-Psicose-3-epimerase (DPE) with recombinant Escherichia coli. High-level production of DPE is one of the key factors in D-Psicose production. In the present study, the gene AAL45544.1 from Agrobacterium tumefaciens str.
Lei, Gu, Juan, Zhang, Baihong, Liu, Chongde, Wu, Guocheng, Du, Jian, Chen   +5 more
openaire   +2 more sources

Immobilization of Agrobacterium tumefaciens d-psicose 3-epimerase onto titanium dioxide for bioconversion of rare sugar

Enzyme and Microbial Technology, 2020
d-Psicose (d-ribo-2-hexulose or d-allulose) is the Carbon-3 epimer of d-fructose sugar and considered as an unnatural (rare) sugar found in low amount in nature. It has about 70% of the relative sweetness but 0.3% of the energy of sucrose, which is suggested as the most suitable sucrose substitute for food additives. Enzymatic biosynthesis using ketose
Samir R, Dedania, Vaibhav K, Patel, Saurabh S, Soni, Darshan H, Patel   +3 more
openaire   +2 more sources

Enhanced production of d‐psicose 3‐epimerase in Bacillus subtilis by regulation of segmented fermentation

Biotechnology and applied biochemistry, 2019
d‐Psicose 3‐epimerase is an enzyme that catalyzes the synthesis of d‐psicose from d‐fructose. We cloned the d‐psicose 3‐epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311.
Gang Fu, Shibin Zhang, Huina Dong, Jingqi Chen, R. Tu, Dawei Zhang   +5 more
semanticscholar   +1 more source

Roles of Ile66 and Ala107 of d-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its substrate, d-fructose

Biotechnology Letters, 2009
Using site-directed mutagenesis, we investigated the roles of Ile66 and Ala107 of D: -psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its true substrate, D: -fructose. When Ile66 was substituted with alanine, glycine, cysteine, leucine, phenylalanine, tryptophan, tyrosine or valine, all the mutants dramatically increased the K (m ...
Hye-Jung, Kim, Byung-Chul, Lim, Soo-Jin, Yeom, Yeong-Su, Kim, Dooil, Kim, Deok-Kun, Oh   +5 more
openaire   +2 more sources

Biochemical characterization of a D-psicose 3-epimerase from Treponema primitia ZAS-1 and its application on enzymatic production of D-psicose.

The Journal of the Science of Food and Agriculture, 2016
Wenli Zhang, Tao Zhang, B. Jiang, W. Mu
semanticscholar   +1 more source

Improving the Thermostability and Catalytic Efficiency of the d-Psicose 3-Epimerase from Clostridium bolteae ATCC BAA-613 Using Site-Directed Mutagenesis.

Journal of Agricultural and Food Chemistry, 2016
Wenli Zhang, Min Jia, Shuhuai Yu, Tao Zhang, Leon Zhou, B. Jiang, W. Mu   +6 more
semanticscholar   +1 more source

Bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on Saccharomyces cerevisiae spores

Journal of Industrial Microbiology & Biotechnology, 2015
Zijie Li, Yi Li, Shenglin Duan, Jia Liu, Peng Yuan, H. Nakanishi, Xiao-Dong Gao   +6 more
semanticscholar   +1 more source

Optimization for allitol production from d-glucose by using immobilized glucose isomerase and recombinant E. coli expressing d-psicose-3-epimerase, ribitol dehydrogenase and formate dehydrogenase

Biotechnology Letters, 2020
Xin Wen, H. Lin, Yilin Ren, Can Li, Chengjia Zhang, Xin Song, Jian-qun Lin, Jianqiang Lin   +7 more
semanticscholar   +1 more source

Characterization of a metal-dependent D-psicose 3-epimerase from a novel strain, Desmospora sp. 8437.

Journal of Agricultural and Food Chemistry, 2013
Wenli Zhang, Dan Fang, Tao Zhang, Leon Zhou, B. Jiang, W. Mu   +5 more
semanticscholar   +1 more source

High-level intra- and extra-cellular production of d-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis

Journal of Industrial Microbiology & Biotechnology, 2016
Jingqi Chen, Yueming Zhu, Gang Fu, Yafeng Song, Zhaoxia Jin, Yuanxia Sun, Dawei Zhang   +6 more
semanticscholar   +1 more source