Results 21 to 30 of about 29,402,319 (134)

Characterization of an Agrobacterium tumefaciens D-psicose 3-epimerase that converts D-fructose to D-psicose. [PDF]

Appl Environ Microbiol, 2006
ABSTRACT The noncharacterized gene previously proposed as the d -tagatose 3-epimerase gene from Agrobacterium tumefaciens was cloned and expressed in Escherichia coli .
Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK.
europepmc   +4 more sources

Correction: Characterization of a Novel Metal-Dependent D-Psicose 3-Epimerase from Clostridium scindens 35704

PLoS ONE, 2014
doaj   +2 more sources

Improvement in the thermostability of D-psicose 3-epimerase from Agrobacterium tumefaciens by random and site-directed mutagenesis. [PDF]

Appl Environ Microbiol, 2011
The S213C, I33L, and I33L S213C variants of d -psicose 3-epimerase from Agrobacterium tumefaciens , which were obtained by random and site-directed mutagenesis, displayed increases of 2.5, 5, and 7.5°C in ...
Choi JG, Ju YH, Yeom SJ, Oh DK.
europepmc   +2 more sources

Construction of hyperthermostable d-allulose 3-epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus. [PDF]

FEBS Open Bio
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Shimada K, Ohtani K, Gullapalli PK, Ishikawa K.   +3 more
europepmc   +2 more sources

Safety of D-allulose as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]

EFSA J
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on D‐allulose as a novel food (NF) pursuant to Regulation (EU) 2015/2283. During the risk assessment, the Panel identified a number of data gaps, which needed to be addressed by the applicant ...
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Turck D, Cámara M, Bohn T, Castenmiller J, De Henauw S, Jos Á, Maciuk A, Mangelsdorf I, McNulty B, Naska A, Pentieva K, Siani A, Thies F, Aguilera-Gómez M, Cubadda F, Frenzel T, Heinonen M, Knutsen HK, McArdle HJ, Neuhäuser-Berthold M, Poulsen M, Maradona MP, Schlatter JR, Siskos A, van Loveren H, Multari S, Rossi A, Hirsch-Ernst KI.   +29 more
europepmc   +2 more sources

Construction and Process Optimization of Cell Factory for Allitol Synthesis with Multienzymatic Cascade System(多酶级联系统制备蒜糖醇的细胞工厂构建及工艺优化) [PDF]

Shipin kexue jishu xuebao, 2022
Allitol is generally used as a novel functional sweetener with great potential applications in food, medical care and other fields. Based on the Izumoring strategy, a multienzymatic cascade system involved glucose isomerase, D-psicose 3-epimerase and ...
ZHAO Jingyi(赵婧邑), GUO Yan(郭燕), FENG Tingting(冯婷婷), CHEN Jing(陈静), SUN Qinju(孙钦菊), LIU Jidong(刘继栋)   +5 more
doaj   +1 more source

Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems

ChemElectroChem, Volume 10, Issue 22, November 14, 2023., 2023
An electrochemically coupled ATP regeneration by pyruvate oxidase and acetate kinase for the phosphorylation of mevalonate was established and expanded by a polyphosphate kinase. The reaction was characterized and compared with other ATP regenerating systems in terms of the phosphate donor properties and biocatalytic metrics.
Regine Siedentop, Tobias Prenzel, Siegfried R. Waldvogel, Katrin Rosenthal, Stephan Lütz   +4 more
wiley   +1 more source

An Update: Enzymatic Synthesis for Industrial Applications. [PDF]

Angew Chem Int Ed Engl
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Bayer T, Wu S, Snajdrova R, Baldenius K, Bornscheuer UT.   +4 more
europepmc   +2 more sources

Food manufacturing processes and technical data used in the exposure assessment of food enzymes

EFSA Journal, Volume 21, Issue 7, July 2023., 2023
Abstract Food enzymes are used for technical purposes in the production of food ingredients or foods‐as‐consumed. In the European Union, the safety of a food enzyme is evaluated by EFSA on the basis of a technical dossier provided by an applicant. Dietary exposure is an integral part of the risk assessment of food enzymes.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Claude Lambré, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk van Loveren, Laurence Vernis, Holger Zorn, Yrjö Roos, Kyriaki Apergi, Daniele Cavanna, Yi Liu, Francesco Pesce, Giulio di Piazza, Rita Ferreira de Sousa, Andrew Chesson   +25 more
wiley   +1 more source

Gene Cloning, Structural Prediction, and Prokaryotic Expression of Agrobacterium tumefaciens D-Psicose-3-Epimerase(根癌农杆菌D-阿洛酮糖-3-差向异构酶基因克隆、结构预测及原核表达) [PDF]

Shipin kexue jishu xuebao, 2019
D-Psicose can be converted from D-fructose catalyzed by D-psicose-3-epimerase. To realize the heterologous expression of D-psicose-3-epimerase (DPEase), the primers were designed to clone and isolate the gene.
ZHU Xingxing(朱星星), YANG Peizhou(杨培周), DU Mingrui(杜明睿), WU Yun(吴芸), JIANG Shaotong(姜绍通)   +4 more
doaj   +1 more source