Results 21 to 30 of about 1,864,743 (129)

Safety of D-allulose as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]

EFSA J
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on D‐allulose as a novel food (NF) pursuant to Regulation (EU) 2015/2283. During the risk assessment, the Panel identified a number of data gaps, which needed to be addressed by the applicant ...
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Turck D, Cámara M, Bohn T, Castenmiller J, De Henauw S, Jos Á, Maciuk A, Mangelsdorf I, McNulty B, Naska A, Pentieva K, Siani A, Thies F, Aguilera-Gómez M, Cubadda F, Frenzel T, Heinonen M, Knutsen HK, McArdle HJ, Neuhäuser-Berthold M, Poulsen M, Maradona MP, Schlatter JR, Siskos A, van Loveren H, Multari S, Rossi A, Hirsch-Ernst KI.   +29 more
europepmc   +2 more sources

A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker. [PDF]

Bioresour Bioprocess, 2017
Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis.In this study,
Chen J, Jin Z, Gai Y, Sun J, Zhang D.
europepmc   +5 more sources

Characterization of d-tagatose-3-epimerase from Rhodobacter sphaeroides that converts d-fructose into d-psicose

Biotechnology Letters, 2009
A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and decreased for other substrates in the order: D-tagatose, D-psicose,
Longtao, Zhang, Wanmeng, Mu, Bo, Jiang, Tao, Zhang   +3 more
core   +4 more sources

Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of d-fructose into d-psicose through fermentation

Enzyme and Microbial Technology, 2020
d-Psicose, as important rare sugar and epimer of d-fructose on the C-3 position, displays unique health benefits and physiological functions in various fields. The production of d-psicose is currently obtained via enzymatic bioconversion, mostly with of d-tagatose 3-epimerase.
Jun Zhang, Chao Xu, Xiaoyan Chen, Xi Ruan, Yu Zhang, Huijuan Xu, Ying Guo, Jingliang Xu, Pengmei Lv, Zhongming Wang   +9 more
openaire   +4 more sources

An Update: Enzymatic Synthesis for Industrial Applications. [PDF]

Angew Chem Int Ed Engl
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Bayer T, Wu S, Snajdrova R, Baldenius K, Bornscheuer UT.   +4 more
europepmc   +2 more sources

Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli [PDF]

Scientific Reports
D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector ...
Haoran Liu, Kang Xu, ShuQi Sun, Yinbiao Wan, Bojia Zhang, Yang Song, Chuanzhuang Guo, Songsen Sui, Ruiming Wang, Piwu Li, Junqing Wang, Zhenshang Xu, Ting Wang   +12 more
doaj   +2 more sources

Toxicological Assessment of D-Allulose From a Novel One-Step Fermentation Process Using Genetically Modified <i>Escherichia coli</i>: A 90-Day Dietary Toxicity Study in Rats. [PDF]

J Toxicol
D‐Allulose is a rare monosaccharide structurally similar to D‐fructose, characterized by low caloric content and relatively high sweetness. This study aimed to conduct a 90‐day oral toxicity test to systemically evaluate the potential toxicological effects of D‐allulose produced via a novel one‐step fermentation process using genetically engineered ...
Li Z, Nie Y, Du H, Zhang W, Zheng S, Cong W, Feng Y, Li G, Jing H, Ning J, Gao S.   +10 more
europepmc   +2 more sources

Correction: Characterization of a Novel Metal-Dependent D-Psicose 3-Epimerase from Clostridium scindens 35704

PLoS ONE, 2014
doaj   +2 more sources

Roles of Ile66 and Ala107 of d-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its substrate, d-fructose

Biotechnology Letters, 2009
Using site-directed mutagenesis, we investigated the roles of Ile66 and Ala107 of D: -psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its true substrate, D: -fructose. When Ile66 was substituted with alanine, glycine, cysteine, leucine, phenylalanine, tryptophan, tyrosine or valine, all the mutants dramatically increased the K (m ...
Hye-Jung, Kim, Byung-Chul, Lim, Soo-Jin, Yeom, Yeong-Su, Kim, Dooil, Kim, Deok-Kun, Oh   +5 more
openaire   +3 more sources

Correction: Characterization of a Novel Metal-Dependent D-Psicose 3-Epimerase from 35704.

PLoS ONE, 2014
Figure 2 in the article, which reports the overexpression and purification of Clostridium scindens DPEase (previously named D-tagatose 3-epimerase) is duplicate from the authors' publication in Food and Fermentation Industries below: Xing Q, Mu W, Jiang B, Zhou L, Zhang T (2011) Cloning, expression, purification and characterization of D-tagatose 3 ...
Wenli Zhang, Dan Fang, Qingchao Xing, Leon Zhou, Bo Jiang, Wanmeng Mu   +5 more
doaj   +3 more sources