Results 21 to 30 of about 509 (137)

Characterization of an Agrobacterium tumefaciens D-psicose 3-epimerase that converts D-fructose to D-psicose. [PDF]

Appl Environ Microbiol, 2006
ABSTRACT The noncharacterized gene previously proposed as the d -tagatose 3-epimerase gene from Agrobacterium tumefaciens was cloned and expressed in Escherichia coli .
Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK.
europepmc   +4 more sources

Assessment of the potential risks in SD rats gavaged with genetically modified yeast containing the cp4-epsps gene. [PDF]

Front Vet Sci
IntroductionDespite the absence of definitive evidence indicating that the cp4-epsps gene and its resultant recombinant proteins have significant harmful effects on either human or animal health, the safety assessment of genetically modified (GM) crops ...
Bi B, Fu X, Jian X, Zhang Y, Jiang Y, Zhou W, Zhao H.   +6 more
europepmc   +3 more sources

Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application. [PDF]

PLoS One
D-Allulose 3-epimerase (DAEase) is the key enzyme catalyzing D-fructose to catalyze into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a substitute ...
Lischer K, Laksmi FA, Nugraha Y, Helbert, Az-Zahra F, Herawan D, Juanssilfero AB, Wibowo DS, Ramadhan KP, Nuryana I, Ali MSM.   +10 more
europepmc   +3 more sources

Construction of hyperthermostable d-allulose 3-epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus. [PDF]

FEBS Open Bio
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Shimada K, Ohtani K, Gullapalli PK, Ishikawa K.   +3 more
europepmc   +2 more sources

Safety of D-allulose as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]

EFSA J
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on D‐allulose as a novel food (NF) pursuant to Regulation (EU) 2015/2283. During the risk assessment, the Panel identified a number of data gaps, which needed to be addressed by the applicant ...
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Turck D, Cámara M, Bohn T, Castenmiller J, De Henauw S, Jos Á, Maciuk A, Mangelsdorf I, McNulty B, Naska A, Pentieva K, Siani A, Thies F, Aguilera-Gómez M, Cubadda F, Frenzel T, Heinonen M, Knutsen HK, McArdle HJ, Neuhäuser-Berthold M, Poulsen M, Maradona MP, Schlatter JR, Siskos A, van Loveren H, Multari S, Rossi A, Hirsch-Ernst KI.   +29 more
europepmc   +2 more sources

Construction and Process Optimization of Cell Factory for Allitol Synthesis with Multienzymatic Cascade System(多酶级联系统制备蒜糖醇的细胞工厂构建及工艺优化) [PDF]

Shipin kexue jishu xuebao, 2022
Allitol is generally used as a novel functional sweetener with great potential applications in food, medical care and other fields. Based on the Izumoring strategy, a multienzymatic cascade system involved glucose isomerase, D-psicose 3-epimerase and ...
ZHAO Jingyi(赵婧邑), GUO Yan(郭燕), FENG Tingting(冯婷婷), CHEN Jing(陈静), SUN Qinju(孙钦菊), LIU Jidong(刘继栋)   +5 more
doaj   +1 more source

Rare sugars: recent advances and their potential role in sustainable crop protection [PDF]

, 2021
Rare sugars are monosaccharides with a limited availability in the nature and almost unknown biological functions. The use of industrial enzymatic and microbial processes greatly reduced their production costs, making research on these molecules more ...
Aziz, Aziz, Aït Barka, Essaid, Mijailovic, Nikola, Nesler, Andrea, Perazzolli, Michele   +4 more
core   +2 more sources

An Update: Enzymatic Synthesis for Industrial Applications. [PDF]

Angew Chem Int Ed Engl
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Bayer T, Wu S, Snajdrova R, Baldenius K, Bornscheuer UT.   +4 more
europepmc   +2 more sources

Expression of d-psicose-3-epimerase from Clostridium bolteae and Dorea sp. and whole-cell production of d-psicose in Bacillus subtilis [PDF]

Annals of Microbiology, 2020
Abstract Purpose d-psicose-3-epimerase (DPEase) catalyses the isomerisation of d-fructose to d-psicose, a rare sugar in nature with unique nutritional and biological functions. An effective industrial-scale method is needed for d-psicose production.
Hongbei Wei, Ruoxuan Zhang, Leyi Wang, Donglong Li, Fangxue Hang, Jidong Liu   +5 more
openaire   +1 more source

Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems

ChemElectroChem, Volume 10, Issue 22, November 14, 2023., 2023
An electrochemically coupled ATP regeneration by pyruvate oxidase and acetate kinase for the phosphorylation of mevalonate was established and expanded by a polyphosphate kinase. The reaction was characterized and compared with other ATP regenerating systems in terms of the phosphate donor properties and biocatalytic metrics.
Regine Siedentop, Tobias Prenzel, Siegfried R. Waldvogel, Katrin Rosenthal, Stephan Lütz   +4 more
wiley   +1 more source