Results 21 to 30 of about 277 (107)
Enhanced Thermostability of D-Psicose 3-Epimerase from Clostridium bolteae through Rational Design and Engineering of New Disulfide Bridges. [PDF]
Int J Mol Sci, 2021 D-psicose 3-epimerase (DPEase) catalyzes the isomerization of D-fructose to D-psicose (aka D-allulose, a low-calorie sweetener), but its industrial application has been restricted by the poor thermostability of the naturally available enzymes. Computational rational design of disulfide bridges was used to select potential sites in the protein structure
Zhao J +5 more
europepmc +4 more sources
Correction: Characterization of a Novel Metal-Dependent D-Psicose 3-Epimerase from 35704.
PLoS ONE, 2014 Figure 2 in the article, which reports the overexpression and purification of Clostridium scindens DPEase (previously named D-tagatose 3-epimerase) is duplicate from the authors' publication in Food and Fermentation Industries below: Xing Q, Mu W, Jiang B, Zhou L, Zhang T (2011) Cloning, expression, purification and characterization of D-tagatose 3 ...
Wenli Zhang +5 more
doaj +3 more sources
Construction and Process Optimization of Cell Factory for Allitol Synthesis with Multienzymatic Cascade System(多酶级联系统制备蒜糖醇的细胞工厂构建及工艺优化) [PDF]
Shipin kexue jishu xuebao, 2022 Allitol is generally used as a novel functional sweetener with great potential applications in food, medical care and other fields. Based on the Izumoring strategy, a multienzymatic cascade system involved glucose isomerase, D-psicose 3-epimerase and ...
ZHAO Jingyi(赵婧邑) +5 more
doaj +1 more source
Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. [PDF]
Protein Cell, 2012 D-psicose 3-epimerase (DPEase) is demonstrated to be useful in the bioproduction of D-psicose, a rare hexose sugar, from D-fructose, found plenty in nature. Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally ...
Chan HC +9 more
europepmc +4 more sources
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems
ChemElectroChem, Volume 10, Issue 22, November 14, 2023., 2023 An electrochemically coupled ATP regeneration by pyruvate oxidase and acetate kinase for the phosphorylation of mevalonate was established and expanded by a polyphosphate kinase. The reaction was characterized and compared with other ATP regenerating systems in terms of the phosphate donor properties and biocatalytic metrics.
Regine Siedentop +4 more
wiley +1 more source
Food manufacturing processes and technical data used in the exposure assessment of food enzymes
EFSA Journal, Volume 21, Issue 7, July 2023., 2023 Abstract Food enzymes are used for technical purposes in the production of food ingredients or foods‐as‐consumed. In the European Union, the safety of a food enzyme is evaluated by EFSA on the basis of a technical dossier provided by an applicant. Dietary exposure is an integral part of the risk assessment of food enzymes.
EFSA Panel on Food Contact Materials +25 more
wiley +1 more source
Gene Cloning, Structural Prediction, and Prokaryotic Expression of Agrobacterium tumefaciens D-Psicose-3-Epimerase(根癌农杆菌D-阿洛酮糖-3-差向异构酶基因克隆、结构预测及原核表达) [PDF]
Shipin kexue jishu xuebao, 2019 D-Psicose can be converted from D-fructose catalyzed by D-psicose-3-epimerase. To realize the heterologous expression of D-psicose-3-epimerase (DPEase), the primers were designed to clone and isolate the gene.
ZHU Xingxing(朱星星) +4 more
doaj +1 more source
Molecular Ecology, Volume 32, Issue 3, Page 703-723, February 2023., 2023 Abstract Microbes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus, one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome.
Hetty KleinJan +10 more
wiley +1 more source
Biotechnology and Bioengineering, Volume 119, Issue 10, Page 2609-2638, October 2022., 2022 Abstract Over the past decade, nanotechnology has been developed and employed across various entities. Among the numerous nanostructured material types, enzyme‐incorporated nanomaterials have shown great potential in various fields, as an alternative to biologically derived as well as synthetically developed hybrid structures.
Shamini Anboo +6 more
wiley +1 more source
EFSA Journal, Volume 20, Issue 7, July 2022., 2022 Abstract The qualified presumption of safety (QPS) approach was developed to provide a regularly updated generic pre‐evaluation of the safety of microorganisms, intended for use in the food or feed chains, to support the work of EFSA's Scientific Panels.
EFSA Panel on Biological Hazards (BIOHAZ) +28 more
wiley +1 more source