Results 41 to 50 of about 509 (137)

Preparation of a Flower-Like Immobilized D-Psicose 3-Epimerase with Enhanced Catalytic Performance [PDF]

Catalysts, 2018
In this present study, we proposed a smart biomineralization method for creating hybrid organic–inorganic nanoflowers using a Co2+-dependent enzyme (D-psicose 3-epimerase; DPEase) as the organic component and cobalt phosphate as the inorganic component. The prepared nanoflowers have many separated petals that have a nanometer size.
Zheng, Lu, Sun, Yining, Wang, Jing, Huang, He, Geng, Xin, Tong, Yi, Wang, Zhi   +6 more
openaire   +1 more source

Conversion of xylan by recyclable spores of Bacillus subtilis displaying thermophilic enzymes [PDF]

, 2017
Background: The Bacillus subtilis spore has long been used to display antigens and enzymes. Spore display can be accomplished by a recombinant and a non-recombinant approach, with the latter proved more efficient than the recombinant one.
Cangiano, Giuseppina, Cobucci-Ponzano, Beatrice, IACONO, ROBERTA, Isticato, Rachele, MATTOSSOVICH, ROSANNA, Moracci, Marco, Ricca, Ezio   +6 more
core   +2 more sources

6-Deoxyhexoses froml-Rhamnose in the Search for Inducers of the Rhamnose Operon: Synergy of Chemistry and Biotechnology [PDF]

, 2016
In the search for alternative non‐metabolizable inducers in the l ‐rhamnose promoter system, the synthesis of fifteen 6‐deoxyhexoses from l ‐rhamnose demonstrates the value of synergy between biotechnology and chemistry.
Estévez, Amalia, Estévez, Ramón J., Fleet, George W. J., Heap, John T., Izumori, Ken, Jenkinson, Sarah F., Kato, Atsushi, Kelly, Ciarán, Liu, Zilei, Marqvorsen, Mikkel H. S., Otero, José M., Wormald, Mark R., Yoshihara, Akihide   +12 more
core   +3 more sources

Purification and Characterization of D-psicose 3 Epimerase (DPEase) From Escherichia coli BL21 (DE3) pET21b dpe

Pakistan Journal of Biological Sciences, 2020
The DPEase enzyme from Agrobacterium tumefaciens is more efficient and has a high activity in D-fructose. The dpe gene has been successfully cloned to Escherichia coli BL21 (DE3) pET-21b dpe but the enzyme has not been purified and its character is unknown. The intent of this study was to purify and assign of DPEase enzyme by recombinant E.
Deby, EdyLiani, Yurnaliza, Yurnaliza, Budi, Saksono   +2 more
openaire   +2 more sources

Exploring the Diversity of Sugar Compounds in Healthy, Prediabetic, and Diabetic Volunteers

Molecular Nutrition &Food Research, Volume 64, Issue 9, May 2020., 2020
A pilot study with a broad sugar profiling analysis during an oral glucose tolerance test (OGTT) in the healthy and diabetic state is performed. Overall, 40 sugar compounds, including rare sugars, are detected in human plasma. A range of sugars reveal similar characteristic changes as glucose during an OGTT.
Carina I. Mack, Paola G. Ferrario, Christoph H. Weinert, Björn Egert, Anja S. Hoefle, Yu‐Mi Lee, Thomas Skurk, Sabine E. Kulling, Hannelore Daniel   +8 more
wiley   +1 more source

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 11: suitability of taxonomic units notified to EFSA until September 2019

EFSA Journal, Volume 18, Issue 2, February 2020., 2020
Abstract Qualified presumption of safety (QPS) was developed to provide a generic safety evaluation for biological agents to support EFSA's Scientific Panels. The taxonomic identity, body of knowledge, safety concerns and antimicrobial resistance are assessed.
EFSA Panel on Biological Hazards (BIOHAZ), Kostas Koutsoumanis, Ana Allende, Avelino Alvarez‐Ordóñez, Declan Bolton, Sara Bover‐Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernández Escámez, Miguel Prieto Maradona, Amparo Querol, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Sandra Correia, Lieve Herman   +26 more
wiley   +1 more source

Rational Design to Improve D-Psicose-3-epimerase Thermal Stability of Blautia wexlerae

Shipin gongye ke-ji
To develop a novel D-psicose-3-epimerase possessing higher thermal stability, an exploration was carried out on the genome of the intestinal probiotic Blautia wexlerae.
Kaixuan RAN, Mingqiang XUN, Kaixin LIN, Yu FU, Lili WANG, Chunxiao ZHANG, Hong YI   +6 more
doaj   +1 more source

Synthesis of allitol from D-psicose using ribitol dehydrogenase and formate dehydrogenase [PDF]

, 2017
Purpose: To synthesize allitol from D-psicose by a combination of novel ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) under optimised production conditions.Methods: RDH and FDH genes were cloned and introduced into pET-22b(+) vectors for ...
Elferga, Ammar, Hassanin, Hinawi A.M., Jiang, Bo, Koko, Marwa Y.F., Letsididi, Rebaone, Mu, Wanmeng   +5 more
core   +2 more sources

Demonstration of Physical Phenomenas and Scavenging Activity from D-Psicose and Methionine Maillard Reaction Products [PDF]

, 2017
Maillard reaction has been well understood as a non-enzymatic reaction between reducing sugars and amino acids to generate the Maillard reaction products (MRPs).
Al-Baarri, Ahmad N, Legowo, Anang M., SUMINAR, Arum Tyas   +2 more
core   +3 more sources

Host-selected mutations converging on a global regulator drive an adaptive leap towards symbiosis in bacteria [PDF]

, 2017
Host immune and physical barriers protect against pathogens but also impede the establishment of essential symbiotic partnerships. To reveal mechanisms by which beneficial organisms adapt to circumvent host defenses, we experimentally evolved ...
Cooper, Vaughn S., Coyle, Matthew, Donner, Rachel A., Foxall, Randi L., Pankey, Molly Sabrina, Perry, Lauren A., Schuster, Brian M., Ster, Ian M., Whistler, Cheryl A.   +8 more
core   +3 more sources