Improvement in the thermostability of D-psicose 3-epimerase from Agrobacterium tumefaciens by random and site-directed mutagenesis. [PDF]
The S213C, I33L, and I33L S213C variants of d -psicose 3-epimerase from Agrobacterium tumefaciens , which were obtained by random and site-directed mutagenesis, displayed increases of 2.5, 5, and 7.5°C in ...
Choi JG, Ju YH, Yeom SJ, Oh DK.
europepmc +2 more sources
Construction and Process Optimization of Cell Factory for Allitol Synthesis with Multienzymatic Cascade System(多酶级联系统制备蒜糖醇的细胞工厂构建及工艺优化) [PDF]
Allitol is generally used as a novel functional sweetener with great potential applications in food, medical care and other fields. Based on the Izumoring strategy, a multienzymatic cascade system involved glucose isomerase, D-psicose 3-epimerase and ...
ZHAO Jingyi(赵婧邑) +5 more
doaj +1 more source
Expression of d-psicose-3-epimerase from Clostridium bolteae and Dorea sp. and whole-cell production of d-psicose in Bacillus subtilis [PDF]
Abstract Purpose d-psicose-3-epimerase (DPEase) catalyses the isomerisation of d-fructose to d-psicose, a rare sugar in nature with unique nutritional and biological functions. An effective industrial-scale method is needed for d-psicose production.
Hongbei Wei +5 more
openaire +1 more source
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems
An electrochemically coupled ATP regeneration by pyruvate oxidase and acetate kinase for the phosphorylation of mevalonate was established and expanded by a polyphosphate kinase. The reaction was characterized and compared with other ATP regenerating systems in terms of the phosphate donor properties and biocatalytic metrics.
Regine Siedentop +4 more
wiley +1 more source
Food manufacturing processes and technical data used in the exposure assessment of food enzymes
Abstract Food enzymes are used for technical purposes in the production of food ingredients or foods‐as‐consumed. In the European Union, the safety of a food enzyme is evaluated by EFSA on the basis of a technical dossier provided by an applicant. Dietary exposure is an integral part of the risk assessment of food enzymes.
EFSA Panel on Food Contact Materials +25 more
wiley +1 more source
Gene Cloning, Structural Prediction, and Prokaryotic Expression of Agrobacterium tumefaciens D-Psicose-3-Epimerase(根癌农杆菌D-阿洛酮糖-3-差向异构酶基因克隆、结构预测及原核表达) [PDF]
D-Psicose can be converted from D-fructose catalyzed by D-psicose-3-epimerase. To realize the heterologous expression of D-psicose-3-epimerase (DPEase), the primers were designed to clone and isolate the gene.
ZHU Xingxing(朱星星) +4 more
doaj +1 more source
Abstract Microbes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus, one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome.
Hetty KleinJan +10 more
wiley +1 more source
Abstract Over the past decade, nanotechnology has been developed and employed across various entities. Among the numerous nanostructured material types, enzyme‐incorporated nanomaterials have shown great potential in various fields, as an alternative to biologically derived as well as synthetically developed hybrid structures.
Shamini Anboo +6 more
wiley +1 more source
Abstract The qualified presumption of safety (QPS) approach was developed to provide a regularly updated generic pre‐evaluation of the safety of microorganisms, intended for use in the food or feed chains, to support the work of EFSA's Scientific Panels.
EFSA Panel on Biological Hazards (BIOHAZ) +28 more
wiley +1 more source
Crystal structure of a novel homodimeric l‐ribulose 3‐epimerase from Methylomonus sp.
d‐Allulose has potential as a low‐calorie sweetener which can suppress fat accumulation. The enzymes capable of d‐allulose production have attracted attention. Here, we report the X‐ray structure of a novel l‐ribulose 3‐epimerase from Methylomonas sp.
Hiromi Yoshida +6 more
wiley +1 more source

