Results 31 to 40 of about 277 (107)

Expression of d-psicose-3-epimerase from Clostridium bolteae and Dorea sp. and whole-cell production of d-psicose in Bacillus subtilis [PDF]

Annals of Microbiology, 2020
Abstract Purpose d-psicose-3-epimerase (DPEase) catalyses the isomerisation of d-fructose to d-psicose, a rare sugar in nature with unique nutritional and biological functions. An effective industrial-scale method is needed for d-psicose production.
Hongbei Wei, Ruoxuan Zhang, Leyi Wang, Donglong Li, Fangxue Hang, Jidong Liu   +5 more
openaire   +1 more source

Crystal structure of a novel homodimeric l‐ribulose 3‐epimerase from Methylomonus sp.

FEBS Open Bio, Volume 11, Issue 6, Page 1621-1637, June 2021., 2021
d‐Allulose has potential as a low‐calorie sweetener which can suppress fat accumulation. The enzymes capable of d‐allulose production have attracted attention. Here, we report the X‐ray structure of a novel l‐ribulose 3‐epimerase from Methylomonas sp.
Hiromi Yoshida, Akihide Yoshihara, Shiro Kato, Susumu Mochizuki, Kazuya Akimitsu, Ken Izumori, Shigehiro Kamitori   +6 more
wiley   +1 more source

Biochemical analysis and the preliminary crystallographic characterization of d-tagatose 3-epimerase from Rhodobacter sphaeroides

Microbial Cell Factories, 2017
Background d-Tagatose 3-epimerase epimerizes d-fructose to yield d-psicose, which is a rare sugar that exists in small quantities in nature and is difficult to synthesize chemically.
Zhengliang Qi, Zhangliang Zhu, Jian-Wen Wang, Songtao Li, Qianqian Guo, Panpan Xu, Fuping Lu, Hui-Min Qin   +7 more
doaj   +1 more source

Exploring the Diversity of Sugar Compounds in Healthy, Prediabetic, and Diabetic Volunteers

Molecular Nutrition &Food Research, Volume 64, Issue 9, May 2020., 2020
A pilot study with a broad sugar profiling analysis during an oral glucose tolerance test (OGTT) in the healthy and diabetic state is performed. Overall, 40 sugar compounds, including rare sugars, are detected in human plasma. A range of sugars reveal similar characteristic changes as glucose during an OGTT.
Carina I. Mack, Paola G. Ferrario, Christoph H. Weinert, Björn Egert, Anja S. Hoefle, Yu‐Mi Lee, Thomas Skurk, Sabine E. Kulling, Hannelore Daniel   +8 more
wiley   +1 more source

Preparation of a Flower-Like Immobilized D-Psicose 3-Epimerase with Enhanced Catalytic Performance [PDF]

Catalysts, 2018
In this present study, we proposed a smart biomineralization method for creating hybrid organic–inorganic nanoflowers using a Co2+-dependent enzyme (D-psicose 3-epimerase; DPEase) as the organic component and cobalt phosphate as the inorganic component. The prepared nanoflowers have many separated petals that have a nanometer size.
Zheng, Lu, Sun, Yining, Wang, Jing, Huang, He, Geng, Xin, Tong, Yi, Wang, Zhi   +6 more
openaire   +1 more source

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 11: suitability of taxonomic units notified to EFSA until September 2019

EFSA Journal, Volume 18, Issue 2, February 2020., 2020
Abstract Qualified presumption of safety (QPS) was developed to provide a generic safety evaluation for biological agents to support EFSA's Scientific Panels. The taxonomic identity, body of knowledge, safety concerns and antimicrobial resistance are assessed.
EFSA Panel on Biological Hazards (BIOHAZ), Kostas Koutsoumanis, Ana Allende, Avelino Alvarez‐Ordóñez, Declan Bolton, Sara Bover‐Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernández Escámez, Miguel Prieto Maradona, Amparo Querol, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Sandra Correia, Lieve Herman   +26 more
wiley   +1 more source

Rational Design to Improve D-Psicose-3-epimerase Thermal Stability of Blautia wexlerae

Shipin gongye ke-ji
To develop a novel D-psicose-3-epimerase possessing higher thermal stability, an exploration was carried out on the genome of the intestinal probiotic Blautia wexlerae.
Kaixuan RAN, Mingqiang XUN, Kaixin LIN, Yu FU, Lili WANG, Chunxiao ZHANG, Hong YI   +6 more
doaj   +1 more source

Characterization of an Agrobacterium tumefaciens D-psicose 3-epimerase that converts D-fructose to D-psicose. [PDF]

Appl Environ Microbiol, 2006
ABSTRACT The noncharacterized gene previously proposed as the d -tagatose 3-epimerase gene from Agrobacterium tumefaciens was cloned and expressed in Escherichia coli .
Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK.
europepmc   +4 more sources

Update: Enzymatische Synthese für industrielle Anwendungen

Angewandte Chemie, Volume 137, Issue 27, July 1, 2025.
Die Biokatalyse hat sich zu einer nachhaltigen und wettbewerbsfähigen Alternative zur herkömmlichen chemischen Synthese entwickelt und ermöglicht die enzymbasierte Herstellung nicht nur von chemischen Grundstoffen, sondern auch von (nicht natürlichen) Aminosäuren, (seltenen) Zuckern und synthetischen Nukleotiden.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Purification and Characterization of D-psicose 3 Epimerase (DPEase) From Escherichia coli BL21 (DE3) pET21b dpe

Pakistan Journal of Biological Sciences, 2020
The DPEase enzyme from Agrobacterium tumefaciens is more efficient and has a high activity in D-fructose. The dpe gene has been successfully cloned to Escherichia coli BL21 (DE3) pET-21b dpe but the enzyme has not been purified and its character is unknown. The intent of this study was to purify and assign of DPEase enzyme by recombinant E.
Deby, EdyLiani, Yurnaliza, Yurnaliza, Budi, Saksono   +2 more
openaire   +2 more sources