Results 81 to 90 of about 286,854 (260)

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Research on Daily Maximum and Minimum Temperature Forecasts by Integrating Terrain Features and Neural Networks [PDF]

Gaoyuan qixiang
Accurate forecasting of daily maximum （T max） and minimum （T min） temperatures is essential for meteorological operational， as enhanced precision safeguards socioeconomic stability in agriculture， transportation and public health.To address pronounced ...
Shu LU, Lin XU, Xue GU, Yue ZHOU, Zejun DAI, Yaqing TAO
doaj   +1 more source

A binormal model of frequency distributions of daily maximum temperature

Australian Meteorological Magazine, 1993
Daily maximum temperature frequency distributions are usually modelled using a single normal distribution. For coastal localities especially, this often provides a poor approximation. Another approach is to represent the distribution as a combination of maritime and continental airstreams with each distinct airstream having a characteristic normal ...
Warwick Grace, Elizabeth Curran
openaire   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Prediction of Summer Daily Maximum Power Load in the Hubei Section of the Yangtze River Economic Belt Based on Meteorological Factors

南方能源建设
[Introduction] This study focuses on the prediction of summer daily maximum power load in Wuhan, Huangshi, and Yichang of the Hubei section in the Yangtze River Economic Belt based on climatic forecast model temperature data.
Lijuan WANG, Yongjian REN, Junchao WANG, Wei OUYANG   +3 more
doaj   +1 more source

The association between local meteorological changes and exacerbation of acute wheezing in Kandy, Sri Lanka

Global Health Action, 2018
Background: Severe wheezing is a common medical emergency. Past studies have demonstrated associations between exacerbation of wheezing and meteorological factors and atmospheric pollution.
N. D. B. Ehelepola, Kusalika Ariyaratne, Amithe Jayaratne   +2 more
doaj   +1 more source

Investigating age and regional effects on the relation between the incidence of heat-related ambulance transport and daily maximum temperature or WBGT. [PDF]

Environ Health Prev Med, 2021
Ueno S, Hayano D, Noguchi E, Aruga T.
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source