Results 121 to 130 of about 25,162,578 (291)
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Apples to Apples? Analog and Digital Data in Curation
Journal of eScience LibrarianshipData collection, especially on paper, started well before the digital age, and there are a few of us in the Data Curation Network (DCN) who continue to have one foot in the analog world.
doaj +2 more sources
The Dacura Data Curation System [PDF]
, 2016 This paper describes the Dacura system which uses linked-data technologies to monitor and constrain the quality of datasets assembled from heter-ogeneous sources and managed by distributed teams of domain experts.
openaire +2 more sources
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
FEBS Open Bio, EarlyView.Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
Data curation technologies and applications
大数据, 2019 Data curation is emerged in order to process,store and applied efficiency.Data curation processes active and continuous management the data through the whole lifecycle of it.And utilizing data curation techniques,data could be used to the maximum extent ...
Minghe YU, Tiezheng NIE, Guoliang LI
doaj
Cell surface CD11c as a neutrophil aging marker molecule
FEBS Open Bio, EarlyView.Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki +5 more
wiley +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source
Digital HealthObjectives Infarct volumes on diffusion-weighted imaging (DWI) are critical for predicting stroke outcomes and guiding late-window endovascular thrombectomy. Although 3D U-Net-based deep learning achieves high sensitivity, it often yields false positives
Hokyu Kim +29 more
doaj +1 more source