De novo assembly and comparative genomics of teleosts
, 2017 During the last 20 years, genome sequencing and assembly projects have changed from requiring large international collaborations to a task that a handful of people can plan and conduct. This has been driven by improvements in sequencing technology and computational methods.
openaire +2 more sources
Long-read de novo genome assembly of Gulf toadfish (Opsanus beta). [PDF]
BMC GenomicsKron NS +3 more
europepmc +1 more source
Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics
Interdisciplinary Medicine, EarlyView.This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li +7 more
wiley +1 more source
Binuclear Copper‐Dependent Oxidative Enzymes Involved in Fungal Natural Product Modifications
Journal of the Chinese Chemical Society, EarlyView.This article summarizes recent biochemical characterizations of a new enzyme family named by the authors as binuclear copper‐dependent oxidative enzymes (BiNCOs). Found in fungal natural product biosynthesis, BiNCOs catalyze diverse CH functionalization reactions, including C(sp3)H halogenation, C(sp3)H hydroxylation, C(sp3)O macrocyclization, and ...
Chen‐Yu Chiang, Masao Ohashi, Yi Tang
wiley +1 more source
Near-complete telomere-to-telomere de novo genome assembly in Egyptian clover (Trifolium alexandrinum). [PDF]
DNA ResSato MP +5 more
europepmc +1 more source
Organizational and Functional Principles of the Obligate Respiratory Chain Supercomplex
Journal of the Chinese Chemical Society, EarlyView.In this review we deliver a comprehensive structural analysis of the actinobacterial respiratory chain supercomplex and discuss the principles underlying the assembly of its supramolecular architecture. ABSTRACT Proton translocating respiratory chain complexes assemble into supramolecular structures in their native cellular environment, yet the ...
Wei‐Chun Kao, Nick Hiatt, Carola Hunte
wiley +1 more source
Snow alga Sanguina aurantia as revealed through de novo genome assembly and annotation. [PDF]
G3 (Bethesda)Raymond BB +3 more
europepmc +1 more source
Ion Activation Methods for Top‐Down Proteomics
Mass Spectrometry Reviews, EarlyView.ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source
De Novo Genome Assembly and Comparative Genome Analysis of the Novel Human Fungal Pathogen Trichosporon austroamericanum Type-Strain CBS 17435. [PDF]
MycopathologiaFrancisco EC +3 more
europepmc +1 more source
Movement Disorders Clinical Practice, EarlyView.Abstract Background Neurodegeneration with Brain Iron Accumulation (NBIA) is a heterogeneous group of heritable, mostly recessive, progressive neurodegenerative diseases characterized by iron deposition in the basal ganglia and brainstem. There are no solid global epidemiological data on prevalence and incidence of NBIA subtypes, but registry data and ...
Susanne A. Schneider +3 more
wiley +1 more source