Results 181 to 190 of about 32,605 (213)

Characteristics of DNA Fractionated on Benzoylated DEAE-Cellulose

Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1975
Chromatography on BD-cellulose columns with a salt gradient and formamide separates cellular DNA into two fractions (fraction I eluted within the salt gradient, fraction II with formamide), the proportions of these two fractions (ca. 2:1) being similar for DNA from a number of eucaryotic organisms.
G, Pirro, H, Feldmann
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Chromatography of acidic glycosaminoglycans on DEAE-cellulose

Journal of Chromatography A, 1972
Abstract An ion-exchange procedure has been developed for the separation and analysis of milligram amounts of acidic glycosaminoglycans. The polysaccharides were first applied to a column of Sephadex G-50 and eluted with 0.15 M sodium chloride.
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Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose

Nature, 1960
THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations1. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day2.
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Molybdate Affects Glucocorticoid Receptor Chromatographyon Deae Cellulose

Journal of Receptor Research, 1986
How molybdate affects the chromatographic behavior of the glucocorticoid receptor on DEAE cellulose was investigated. Over the range 0-20 mM, molybdate caused a progressive change in the elution positions of both the transformed and untransformed receptor. At 20 mM molybdate, the transformed receptor eluted from the column before application of the KCl
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Immobilized Glucose Isomerase on DEAE Cellulose Beads

Starch - Stärke, 1981
AbstractPurified glucose isomerase from Actinoplanes missouriensis was immobilized on porous DEAE‐cellulose beads by simple adsorption. The immobilized glucose isomerase retained over 70% of its original activity. The hinderance of immobilized enzyme activity due to pore diffusion and film diffusion was insignificant with the bead size at 35 mesh or ...
L. F. Chen, C. S. Gong, G. T. Tsao
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Purification of Circular DNA Using Benzoylated Naphthoylated DEAE-Cellulose

DNA, 1985
Un-nicked circular DNA can be separated from protein, RNA, and other DNA in a simple three-step protocol consisting of exonuclease III digestion, extraction with benzoylated naphthoylated DEAE-cellulose (BND cellulose) in 1 M NaCl, and alcohol precipitation of the remaining supercoiled DNA.
H, Gamper, N, Lehman, J, Piette, J E, Hearst   +3 more
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Heparin binding and release properties of DEAE cellulose membranes

Biomaterials, 1983
Two cellulose-based membranes, containing 5 and 20% N, N-diethyl-aminoethyl cellulose (DEAE) respectively, were investigated for ionic attachment of heparin and heparin release. Heparinization was achieved by recirculation of a heparin-containing glycine buffer over each membrane. Heparin was determined by chemical analysis or by 99mTc labelling.
E, Schmitt, M, Holtz, H, Klinkmann, G, Esther, J M, Courtney   +4 more
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A DEAE-cellulose filter disk assay for aminoacyl-tRNA

Analytical Biochemistry, 1974
Abstract A filtration assay is described for quantitation of aminoacylated tRNA. [ 3 H]Aminoacyl-tRNA is adsorbed on DEAE-cellulose filter disks under conditions in which unreacted amino acid is removed by a 100-m m glycine-HCl (pH 2.3) wash. The assay is rapid, simple to perform, and linear over the range of 0.005–20 A 260 units/50-μl filtered ...
D V, Santi, R T, Anderson
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The purification of phosphate esters on DEAE-cellulose

Journal of Chromatography A, 1971
Abstract The utility of DEAE-cellulose for separating phosphate esters is examined. Emphasis is given to the fractionation of labile derivatives which cannot readily be isolated by conventional methods. The factors involved in resolving these phosphate derivatives from contaminants such as inorganic phosphate, sulphate, chloride and free sugars on ...
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Assay for phosphatidylserine decarboxylase utilizing DEAE-cellulose column chromatography

Analytical Biochemistry, 1989
A simple assay for phosphatidylserine decarboxylase is described. Following incubation of a mitochondrial fraction from Saccharomyces cerevisiae with purified, exogenous phosphatidyl[3H]serine, the lipid extract is applied to a small DEAE-cellulose column equilibrated in CHCI3-CH3OH (1:1).
J H, Overmeyer, C J, Waechter
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