A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication [PDF]
mBio, 2022 Decapping enzymes remove the 5′ cap of eukaryotic mRNA, leading to accelerated RNA decay. They are critical in regulating RNA homeostasis and play essential roles in many cellular and life processes.
Shuai Cao +4 more
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Dcp2 C-terminal cis-binding elements control selective targeting of the decapping enzyme by forming distinct decapping complexes [PDF]
eLife, 2022 A single Dcp1–Dcp2 decapping enzyme targets diverse classes of yeast mRNAs for decapping-dependent 5′ to 3′ decay, but the molecular mechanisms controlling mRNA selectivity by the enzyme remain elusive.
Feng He, Chan Wu, Allan Jacobson
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miR-4293 upregulates lncRNA WFDC21P by suppressing mRNA-decapping enzyme 2 to promote lung carcinoma proliferation [PDF]
Cell Death and Disease, 2021 Non-coding RNAs (ncRNAs) involve in diverse biological processes by post-transcriptional regulation of gene expression. Emerging evidence shows that miRNA-4293 plays a significant role in the development of non-small cell lung cancer.
Qian Zhang +14 more
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The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes. [PDF]
PLoS Pathogens, 2017 5'-3' decay is the major mRNA decay pathway in many eukaryotes, including trypanosomes. After deadenylation, mRNAs are decapped by the nudix hydrolase DCP2 of the decapping complex and finally degraded by the 5'-3' exoribonuclease. Uniquely, trypanosomes
Susanne Kramer
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Effect of the mRNA decapping enzyme scavenger (DCPS) inhibitor RG3039 on glioblastoma [PDF]
Journal of Translational MedicineBackground Patients with glioblastoma (GBM) have a poor prognosis and limited treatment options. The mRNA decapping enzyme scavenger (DCPS) is a cap-hydrolyzing enzyme.
Hao Duan +17 more
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Conserved and divergent features of human mRNA decapping revealed by biochemical reconstitution [PDF]
Nature CommunicationsDecapping is a critical step in mRNA decay, but the mechanisms regulating human decapping enzyme DCP2 remain poorly understood. Here, we reconstitute the human decapping network using full-length recombinant proteins and compare it to the yeast system ...
Eric A. J. Simko +4 more
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysis [PDF]
Structure, 2022 Jacek Jemielity +2 more
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Biochemical and functional characterization of orf virus decapping protein OV71 [PDF]
Virology JournalBackground Nudix enzymes constitute a family of hydrolases that share a conserved Nudix motif, which catalyzes the hydrolysis of nucleoside diphosphates linked to another moiety X.
Mandanda N. Mthethwa +8 more
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Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genes [PDF]
eLifeA large fraction of newly transcribed RNA is degraded in the nucleus, but nuclear mRNA degradation pathways remain largely understudied. The yeast nuclear endoribonuclease Rnt1 has a well-characterized role in the maturation of many ncRNA precursors ...
Lee-Ann Notice-Sarpaning +4 more
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Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2017 Remco Sprangers, Jan Philip Wurm
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