General decapping activators target different subsets of inefficiently translated mRNAs
eLife, 2018 The Dcp1-Dcp2 decapping enzyme and the decapping activators Pat1, Dhh1, and Lsm1 regulate mRNA decapping, but their mechanistic integration is unknown. We analyzed the gene expression consequences of deleting PAT1, LSM1, or DHH1, or the DCP2 C-terminal ...
Feng He +3 more
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Pby1 is a direct partner of the Dcp2 decapping enzyme. [PDF]
Nucleic Acids Res, 2020 International audienceMost eukaryotic mRNAs harbor a characteristic 5 m 7 GpppN cap that promotes pre-mRNA splicing, mRNA nucleocytoplasmic transport and translation while also protecting mRNAs from exonucleolytic attacks.
Charenton C +6 more
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Fluorescence-Based Activity Screening Assay Reveals Small Molecule Inhibitors of Vaccinia Virus mRNA Decapping Enzyme D9. [PDF]
ACS Chem Biol, 2022 [Image: see text] Vaccinia virus (VACV) represents a family of poxviruses, which possess their own decapping machinery as a part of their strategy to eliminate host mRNAs and evade the innate immune response.
Bednarczyk M +9 more
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Structure and function of the bacterial decapping enzyme NudC. [PDF]
Nat Chem Biol, 2016 RNA capping and decapping are thought to be distinctive features of eukaryotes. The redox cofactor NAD was recently discovered to be attached to small regulatory RNAs in bacteria in a cap-like manner, and Nudix hydrolase NudC was found to act as a NAD ...
Höfer K +8 more
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Molecular basis of the selective processing of short mRNA substrates by the DcpS mRNA decapping enzyme. [PDF]
Proc Natl Acad Sci U S A, 2020 The 5' messenger RNA (mRNA) cap structure enhances translation and protects the transcript against exonucleolytic degradation. During mRNA turnover, this cap is removed from the mRNA.
Fuchs AL, Wurm JP, Neu A, Sprangers R.
europepmc +2 more sources
Dcp2 C-terminal Cis-Binding Elements Control Selective Targeting of the Decapping Enzyme by Forming Distinct Decapping Complexes [preprint] [PDF]
, 2021 This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.A single Dcp1-Dcp2 decapping enzyme targets diverse classes of yeast mRNAs for decapping-dependent 5’ to 3’ decay, but the molecular ...
Wu, Chan, Jacobson, Allan, He, Feng
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Eukaryotic mRNA Decapping Activation
Frontiers in Genetics, 2022 The 5′-terminal cap is a fundamental determinant of eukaryotic gene expression which facilitates cap-dependent translation and protects mRNAs from exonucleolytic degradation.
Elva Vidya +3 more
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Dcp2: an mRNA decapping enzyme that adopts many different shapes and forms.
Curr Opin Struct Biol, 2019 Eukaryotic mRNAs contain a 5' cap structure that protects the transcript against rapid exonucleolytic degradation. The regulation of cellular mRNA levels therefore depends on a precise control of the mRNA decapping pathways.
Wurm JP, Sprangers R.
europepmc +2 more sources
Is mRNA decapping by ApaH like phosphatases present in eukaryotes beyond the Kinetoplastida?
BMC Ecology and Evolution, 2021 Background ApaH like phosphatases (ALPHs) originate from the bacterial ApaH protein and have been identified in all eukaryotic super-groups. Only two of these proteins have been functionally characterised.
Paula Andrea Castañeda Londoño +3 more
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Investigation of IRES Insertion into the Genome of Recombinant MVA as a Translation Enhancer in the Context of Transcript Decapping. [PDF]
PLoS ONE, 2015 Recombinant modified vaccinia virus Ankara (MVA) has been used to deliver vaccine candidate antigens against infectious diseases and cancer. MVA is a potent viral vector for inducing high magnitudes of antigen-specific CD8+ T cells; however the cellular ...
Naif Khalaf Alharbi +3 more
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