Eukaryotic mRNA Decapping Activation [PDF]
Frontiers in Genetics, 2022 The 5′-terminal cap is a fundamental determinant of eukaryotic gene expression which facilitates cap-dependent translation and protects mRNAs from exonucleolytic degradation.
Thomas Duchaine, Elva Vidya
exaly +4 more sources
Is mRNA decapping by ApaH like phosphatases present in eukaryotes beyond the Kinetoplastida? [PDF]
BMC Ecology and Evolution, 2021 Background ApaH like phosphatases (ALPHs) originate from the bacterial ApaH protein and have been identified in all eukaryotic super-groups. Only two of these proteins have been functionally characterised.
Paula Andrea Castañeda Londoño +3 more
doaj +2 more sources
Selective Destabilization of Transcripts by mRNA Decapping Regulates Oocyte Maturation and Innate Immunity Gene Expression during Ageing in C. elegans [PDF]
Biology, 2023 Removal of the 5′ cap structure of RNAs (termed decapping) is a pivotal event in the life of cytoplasmic mRNAs mainly catalyzed by a conserved holoenzyme, composed of the catalytic subunit DCP2 and its essential cofactor DCP1.
Fivos Borbolis +6 more
doaj +2 more sources
The mRNA decapping machinery targets LBD3/ASL9 to mediate apical hook and lateral root development [PDF]
Life Science Alliance, 2023 This study identifies LBD3/ASL9 as targets of mRNA decay machinery, and ASL9 contributes to apical hook and lateral root development, possibly through interfering with cytokinin/auxin responses.
Zhangli Zuo +9 more
doaj +2 more sources
Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b [PDF]
Life Science AllianceThe study represents the first functional dissection of the two human cofactors present in the RNA decapping complex, and, significantly, it evaluates their role in the transcript buffering system.
Ivana Vukovic +6 more
doaj +2 more sources
Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions [PDF]
eLifeThe mRNA 5'-cap structure removal by the decapping enzyme DCP2 is a critical step in gene regulation. While DCP2 is the catalytic subunit in the decapping complex, its activity is strongly enhanced by multiple factors, particularly DCP1, which is the ...
Ting-Wen Chen +7 more
doaj +2 more sources
Conserved and divergent features of human mRNA decapping revealed by biochemical reconstitution [PDF]
Nature CommunicationsDecapping is a critical step in mRNA decay, but the mechanisms regulating human decapping enzyme DCP2 remain poorly understood. Here, we reconstitute the human decapping network using full-length recombinant proteins and compare it to the yeast system ...
Eric A. J. Simko +4 more
doaj +2 more sources
The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes. [PDF]
PLoS Pathogens, 2017 5'-3' decay is the major mRNA decay pathway in many eukaryotes, including trypanosomes. After deadenylation, mRNAs are decapped by the nudix hydrolase DCP2 of the decapping complex and finally degraded by the 5'-3' exoribonuclease. Uniquely, trypanosomes
Susanne Kramer
doaj +2 more sources
mRNA decapping proteins regulate EIN2-dependent ethylene signaling in arabidopsis [PDF]
Frontiers in Plant ScienceEthylene signaling regulates plant growth and stress adaptation through a well-defined pathway, yet its mechanistic complexity remains underexplored. Here, we identify two mRNA decapping proteins, DCP1 and DCP2, as novel regulators of canonical ethylene ...
Bai Hui Jiang +14 more
doaj +2 more sources
Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis [PDF]
Nature Communications, 2018 The decapping enzyme Dcp2 removes the 5′ eukaryotic cap from mRNA transcripts and acts in concert with its essential activator Dcp1 and various coactivators.
Jeffrey S. Mugridge +4 more
doaj +2 more sources