Eukaryotic mRNA Decapping Activation
Frontiers in Genetics, 2022 The 5′-terminal cap is a fundamental determinant of eukaryotic gene expression which facilitates cap-dependent translation and protects mRNAs from exonucleolytic degradation.
Elva Vidyā, Thomas F Duchaine
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Conserved and divergent features of human mRNA decapping revealed by biochemical reconstitution [PDF]
Nature CommunicationsDecapping is a critical step in mRNA decay, but the mechanisms regulating human decapping enzyme DCP2 remain poorly understood. Here, we reconstitute the human decapping network using full-length recombinant proteins and compare it to the yeast system ...
Eric A. J. Simko +4 more
doaj +2 more sources
Biochemical and functional characterization of orf virus decapping protein OV71 [PDF]
Virology JournalBackground Nudix enzymes constitute a family of hydrolases that share a conserved Nudix motif, which catalyzes the hydrolysis of nucleoside diphosphates linked to another moiety X.
Mandanda N. Mthethwa +8 more
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The Rice Stripe Virus p2 Interacts With Lsm1 and Disrupts the Lsm1-Lsm4 Complex to Facilitate the Viral infection. [PDF]
Mol Plant PatholRSV p2 binds Lsm1, disrupting Lsm1‐Lsm4 association and impairing decapping‐dependent mRNA decay in P‐bodies, leading to the accumulation of unstable mRNAs and enhanced RSV infection. ABSTRACT Processing bodies (P‐bodies) play central roles in RNA metabolism and in the regulation of viral infection.
Hu A +12 more
europepmc +2 more sources
Decapping activators Edc3 and Scd6 act redundantly with Dhh1 in post-transcriptional repression of starvation-induced pathways [PDF]
eLifeDegradation of many yeast mRNAs involves decapping by the Dcp1:Dcp2 complex. Previous studies on decapping activators Edc3 and Scd6 suggested their limited roles in mRNA decay.
Rakesh Kumar +7 more
doaj +2 more sources
mRNA decapping: finding the right structures [PDF]
Philosophical Transactions of the Royal Society B: Biological Sciences, 2018 In eukaryotes, the elimination of the m 7 GpppN mRNA cap, a process known as decapping, is a critical, largely irreversible and highly regulated step of mRNA decay that withdraws the targeted mRNAs from the pool of translatable templates. The decapping reaction is catalysed by a multi-protein
Clement Charenton, Marc Graille
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Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions [PDF]
eLifeThe mRNA 5'-cap structure removal by the decapping enzyme DCP2 is a critical step in gene regulation. While DCP2 is the catalytic subunit in the decapping complex, its activity is strongly enhanced by multiple factors, particularly DCP1, which is the ...
Ting-Wen Chen +7 more
doaj +2 more sources
RNA degradation triggered by decapping is largely independent of initial deadenylation
EMBO JournalRNA stability, important for eukaryotic gene expression, is thought to depend on deadenylation rates, with shortened poly(A) tails triggering decapping and 5′ to 3′ degradation.
Lena Audebert +2 more
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CleanCap M6 inhibits decapping of exogenously delivered IVT mRNA [PDF]
Molecular Therapy: Nucleic AcidsCo-transcriptional capping allows exogenous mRNAs to yield robust protein expression. Identifying additional mRNA modifications that further boost protein output will be crucial for developing more efficacious mRNA therapies.
Zachary F. Mandell +13 more
doaj +2 more sources
Creatine mitigates neurogenesis impairment caused by defective DcpS decapping [PDF]
Scientific ReportsBiallelic mutations in the DCPS gene disrupting the decapping activity of the scavenger decapping protein DcpS, leads to neurodevelopmental deficiencies and intellectual disability.
Jun Yang +5 more
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