Results 11 to 20 of about 7,630 (176)
eLife, 2022 A single Dcp1–Dcp2 decapping enzyme targets diverse classes of yeast mRNAs for decapping-dependent 5′ to 3′ decay, but the molecular mechanisms controlling mRNA selectivity by the enzyme remain elusive.
Feng He, Chan Wu, Allan Jacobson
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Biology, 2023 Removal of the 5′ cap structure of RNAs (termed decapping) is a pivotal event in the life of cytoplasmic mRNAs mainly catalyzed by a conserved holoenzyme, composed of the catalytic subunit DCP2 and its essential cofactor DCP1.
Fivos Borbolis +6 more
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eLife, 2023 Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, in coupling codon non-optimality to enhanced degradation, and as a translational repressor, but its functions in ...
Anil Kumar Vijjamarri +11 more
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A unique mRNA decapping complex in trypanosomes [PDF]
Nucleic Acids Research, 2023 AbstractRemoval of the mRNA 5′ cap primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme Dcp2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5′-3′exoribonuclease Xrn1.
Susanne Kramer +16 more
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Transcript-Specific Decapping and Regulated Stability by the Human Dcp2 Decapping Protein [PDF]
Molecular and Cellular Biology, 2008 mRNA decapping is a critical step in the control of mRNA stability and gene expression and is carried out by the Dcp2 decapping enzyme. Dcp2 is an RNA binding protein that must bind RNA in order to recognize the cap for hydrolysis. We demonstrate that human Dcp2 (hDcp2) preferentially binds to a subset of mRNAs and identify sequences at the 5' terminus
You, Li +2 more
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Control of mRNA decapping by autoinhibition [PDF]
Nucleic Acids Research, 2018 5’ mediated cytoplasmic RNA decay is a conserved cellular process in eukaryotes. While the functions of the structured core domains in this pathway are understood, the role of abundant intrinsically disordered regions (IDRs) is lacking. Here we reconstitute the Dcp1:Dcp2 complex containing a portion of the disordered C-terminus and show its activity is
Paquette, David R +3 more
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Targeting an mRNA for Decapping [PDF]
Molecular Cell, 2001 A critical step in the turnover of yeast mRNAs is decapping. Two yeast proteins, Dcp1p and Dcp2p, are absolutely required for decapping, although their precise roles in the decapping reaction have not been established. To determine the function of both Dcp1p and Dcp2p in decapping, we purified recombinant versions of these proteins from Escherichia ...
Tharun, Sundaresan, Parker, Roy
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Targeting mRNA Decapping in AML [PDF]
Cancer Cell, 2018 In this issue of Cancer Cell, Yamauchi et al. identify a dependency of acute myeloid leukemia (AML) on DCPS, which catalyzes the final step of 3'-to-5' mRNA decay and is implicated in numerous aspects of RNA metabolism. DCPS is targetable with a clinical inhibitor, underscoring the translational importance of this discovery.
Akihide, Yoshimi, Omar, Abdel-Wahab
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Interactions between Upf1 and the decapping factors Edc3 and Pat1 in Saccharomyces cerevisiae. [PDF]
PLoS ONE, 2011 In Saccharomyces cerevisiae, mRNA transcripts with premature termination codons are targeted for deadenylation independent decapping and 5' to 3' decay in a quality control pathway termed nonsense-mediated decay (NMD).
Kylie D Swisher, Roy Parker
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The DEAD-box protein Dhh1 promotes decapping by slowing ribosome movement. [PDF]
PLoS Biology, 2012 Translational control and messenger RNA (mRNA) decay represent important control points in the regulation of gene expression. In yeast, the major pathway for mRNA decay is initiated by deadenylation followed by decapping and 5'-3' exonucleolytic ...
Thomas Sweet +2 more
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