Targeted Control of Gene Expression Using CRISPR-Associated Endoribonucleases [PDF]
CellsCRISPR-associated endoribonucleases (Cas RNases) cleave single-stranded RNA in a highly sequence-specific manner by recognizing and binding to short RNA sequences known as direct repeats (DRs).
Sagar J. Parikh +8 more
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Stem-loop structures control mRNA processing of the cellulosomal cip-cel operon in Ruminiclostridium cellulolyticum [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Anaerobic, mesophilic, and cellulolytic Ruminiclostridium cellulolyticum produces an efficient cellulolytic extracellular complex named cellulosome, which consist of a non-catalytic multi-functional integrating subunit, organizing the various ...
Na Wang +4 more
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MBio, 2021 Toxin-antitoxin (TA) systems are widespread genetic modules found in almost all bacteria that can regulate their growth and may play prominent roles in phage defense.
Sarah M Fortune, Michael T Laub
exaly +3 more sources
De novo design of hypercompact transcript degraders by engineering substrate-specific toxins and Cas6-CBS system [PDF]
Nature CommunicationsArtificial assembly of small functional proteins provides effective strategies for development of compact RNA degradation systems, which overcome the challenges associated with delivery.
Pin-Ru Chen +7 more
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5′ DREDGE: Direct Repeat-Enabled Downregulation of Gene Expression via the 5′ UTR of Target Genes [PDF]
CellsDespite the availability of numerous methods for controlling gene expression, there remains a strong need for technologies that maximize two key properties: selectivity and reversibility.
Sagar J. Parikh +6 more
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Molecular basis for the calcium-dependent activation of the ribonuclease EndoU [PDF]
Nature CommunicationsRibonucleases (RNases) are ubiquitous enzymes that process or degrade RNA, essential for cellular functions and immune responses. The EndoU-like superfamily includes endoribonucleases conserved across bacteria, eukaryotes, and certain viruses, with an ...
Florian Malard +8 more
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A Novel Method to Isolate RNase MRP Using RNA Streptavidin Aptamer Tags
Bio-Protocol, 2023 Interactions between RNA-binding proteins and RNA molecules are at the center of multiple biological processes. Therefore, accurate characterization of the composition of ribonucleoprotein complexes (RNPs) is crucial.
Violette Charteau +2 more
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Characterization of MazF-Mediated Sequence-Specific RNA Cleavage in Pseudomonas putida Using Massive Parallel Sequencing. [PDF]
PLoS ONE, 2016 Under environmental stress, microbes are known to alter their translation patterns using sequence-specific endoribonucleases that we call RNA interferases. However, there has been limited insight regarding which RNAs are specifically cleaved by these RNA
Tatsuki Miyamoto +4 more
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Scientific Reports, 2021 Enterohaemorrhagic Escherichia coli (EHEC) comprise a group of intestinal pathogens responsible for a range of illnesses, including kidney failure and neurological compromise.
Patricia B. Lodato
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Fifty Shades of Erns: Innate Immune Evasion by the Viral Endonucleases of All Pestivirus Species
Viruses, 2022 The genus Pestivirus, family Flaviviridae, includes four historically accepted species, i.e., bovine viral diarrhea virus (BVDV)-1 and -2, classical swine fever virus (CSFV), and border disease virus (BDV).
Elena de Martin, Matthias Schweizer
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