Results 31 to 40 of about 5,581 (192)
Mutational and Kinetic Analysis of APE1 Endoribonuclease Activity [PDF]
Molecular Biology, 2021 Human apurinic/apyrimidinic endonuclease 1 (APE1) participates in the DNA repair system. It is believed that the main biological function of APE1 is Mg^(2+)-dependent hydrolysis of AP-sites in DNA. On the base of structural data, kinetic studies, and mutation analysis, the key stages of APE1 interaction with damaged DNA were established.
Kuznetsova, A. A. +4 more
openaire +3 more sources
Synthesis of 5’ end-labeled RNA
Bio-Protocol, 2012 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not
Harald Putzer
doaj +1 more source
Sequence-specific endoribonucleases
Postępy Biochemii, 2016 Ribonucleases are nucleolytic enzymes that commonly occur in living organisms and act by cleaving RNA molecules. These enzymes are involved in basic cellular processes, including the RNA maturation that accompanies the formation of functional RNAs, as well as RNA degradation that enables removal of defective or dangerous molecules or ones that have ...
Dawid, Głów +3 more
openaire +3 more sources
Involvement of the Escherichia coli endoribonucleases G and E in the secondary processing of RegB-cleaved transcripts of bacteriophage T4 [PDF]
, 2008 Sequence-specific endoribonuclease RegB of bacteriophage T4 cleaves early phage mRNAs and facilitates the transition between early and subsequent phases of T4 gene expression.
Truncaite, Lidija +3 more
core +1 more source
Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome
Nature Communications, 2019 MazF endoribonucleases are thought to arrest growth of Mycobacterium tuberculosis by global translation inhibition. Here, Barth et al. show that MazF-mt9 cleaves a specific tRNA, leading to ribosome stalling at AAA codons and thus selective mRNA ...
Valdir C. Barth +5 more
doaj +1 more source
Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803
mLife, 2023 Endoribonucleases govern the maturation and degradation of RNA and are indispensable in the posttranscriptional regulation of gene expression. A key endoribonuclease in Gram‐negative bacteria is RNase E.
Satoru Watanabe +10 more
doaj +1 more source
Substrate specificity of human MCPIP1 endoribonuclease [PDF]
Scientific Reports, 2018 AbstractMCPIP1, also known as Regnase-1, is a ribonuclease crucial for regulation of stability of transcripts related to inflammatory processes. Here, we report that MCPIP1 acts as an endonuclease by degrading several stem-loop RNA structures and single-stranded RNAs.
Mateusz Wilamowski +3 more
openaire +4 more sources
, 2023 The N7-methyl guanosine cap structure is an essential 5′ end modification of eukaryotic mRNA. It plays a critical role in many aspects of the life cycle of mRNA, including nuclear export, stability, and translation. Equipping synthetic transcripts with a
Eric J. Wolf (17201565) +7 more
core +1 more source
Glutathione‐Responsive Acyl‐Modifications for Targeted RNA Decaging and Prolonged Protein Synthesis
Angewandte Chemie, EarlyView.The self‐immolation of disulfide‐based mRNA modifications in response to endogenous glutathione (GSH) promotes a gradual release of translatable mRNA within the cell, leading to improved nuclease resistance, tunable release properties, and a significant increase (up to 600%) of target protein production over time. This strategy offers an exciting proof
Mary E. Flood +6 more
wiley +2 more sources
Structural Biology of the Arterivirus nsp11 Endoribonucleases [PDF]
Journal of Virology, 2017 ABSTRACT Endoribonuclease (NendoU) is unique and conserved as a major genetic marker in nidoviruses that infect vertebrate hosts. Arterivirus nonstructural protein 11 (nsp11) was shown to have NendoU activity and play essential roles in the viral life cycle.
Manfeng Zhang +7 more
openaire +2 more sources