Targeted Control of Gene Expression Using CRISPR-Associated Endoribonucleases [PDF]
CellsCRISPR-associated endoribonucleases (Cas RNases) cleave single-stranded RNA in a highly sequence-specific manner by recognizing and binding to short RNA sequences known as direct repeats (DRs).
Sagar J. Parikh +8 more
doaj +4 more sources
Endoribonucleases associated with RNAs in chick embryos [PDF]
FEBS Letters, 1979 proteins in hemopoietic rat cells were reported to have RNase activities [ 121. Similarly, an endogeneous endoribonucleolytic activity was found in the 50 S ribosomal subunit of Eschenihia coli [ 131. This paper describes the existence of a RNA-bound cytoplasmic endoribonuclease in chick embryo.
C D Denoya, J L la Torre, E A Scodeller
exaly +4 more sources
Stem-loop structures control mRNA processing of the cellulosomal cip-cel operon in Ruminiclostridium cellulolyticum [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Anaerobic, mesophilic, and cellulolytic Ruminiclostridium cellulolyticum produces an efficient cellulolytic extracellular complex named cellulosome, which consist of a non-catalytic multi-functional integrating subunit, organizing the various ...
Na Wang +4 more
doaj +2 more sources
mBio, 2021 Toxin-antitoxin (TA) systems are widespread genetic modules found in almost all bacteria that can regulate their growth and may play prominent roles in phage defense.
Peter H. Culviner +3 more
doaj +3 more sources
A Portable Colorimetric Device for Rapid Bacterial Detection with Cleavable Functional Nucleic Acid Probes for A Common Bacterial Endoribonuclease [PDF]
Angewandte Chemie, Volume 138, Issue 28, 6 July 2026.A simple, rapid, and portable gold‐coated filter tip‐based assay (GFTA) is developed for bacterial detection. The GFTA leverages cleavable functional nucleic acid probes as molecular recognition elements to target RNase H2, a highly conserved bacterial endoribonuclease, enabling sensitive and specific colorimetric detection of bacterial pathogens ...
Jiuxing Li +4 more
wiley +3 more sources
De novo design of hypercompact transcript degraders by engineering substrate-specific toxins and Cas6-CBS system [PDF]
Nature CommunicationsArtificial assembly of small functional proteins provides effective strategies for development of compact RNA degradation systems, which overcome the challenges associated with delivery.
Pin-Ru Chen +7 more
doaj +2 more sources
Characterization of MazF-Mediated Sequence-Specific RNA Cleavage in Pseudomonas putida Using Massive Parallel Sequencing. [PDF]
PLoS ONE, 2016 Under environmental stress, microbes are known to alter their translation patterns using sequence-specific endoribonucleases that we call RNA interferases. However, there has been limited insight regarding which RNAs are specifically cleaved by these RNA
Tatsuki Miyamoto +4 more
doaj +2 more sources
An RNA-seq based comparative approach reveals the transcriptome-wide interplay between 3′-to-5′ exoRNases and RNase Y [PDF]
Nature Communications, 2020 Bacterial RNA degradation is typically initiated by endoribonucleases and followed by exoribonucleases. Here the authors report the targetome of endoRNase Y in Streptococcus pyogenes, revealing the interplay between RNase Y and 3′-to-5′ exoribonuclease ...
Laura Broglia +6 more
doaj +2 more sources
Processing Endoribonucleases and mRNA Degradation in Bacteria [PDF]
Journal of Bacteriology, 2002 Volume 184, no. 17, p. 4645-4657, 2002. Page 4647, column 1, lines 42-45: The sentence should read “For example, one message in the T7 early polycistronic mRNA, as well as a few late messages, yields increased protein from RNase III cleavage while the others are not affected by such cleavages (41).”
David Kennell
openaire +4 more sources
5′ DREDGE: Direct Repeat-Enabled Downregulation of Gene Expression via the 5′ UTR of Target Genes [PDF]
CellsDespite the availability of numerous methods for controlling gene expression, there remains a strong need for technologies that maximize two key properties: selectivity and reversibility.
Sagar J. Parikh +6 more
doaj +2 more sources