Results 81 to 90 of about 23,224 (220)
Alterations in antigenic structure of gonadotropins following deglycosylation
Radioimmunological techniques were utilized to probe possible changes in conformation of gonadotropins (human chorionic gonadotropin-hCG; and ovine luteinizing hormone—oLH) following chemical deglycosylation (DG-hCG and DG-LH). All antisera produced in rabbits, rats or mice contained antibodies that were specific to the deglycosylated hormones with the
M R, Sairam, J, Linggen, G N, Bhargavi
openaire +2 more sources
The Arabidopsis NPF7.2 mediates coumarin uptake for root iron acquisition
Summary Iron (Fe) is a transition metal necessary for achieving essential physiological processes throughout the plant lifecycle. In Arabidopsis thaliana, secreting Fe‐mobilizing coumarins (FMC) is a key mechanism enabling roots to acquire nonbioavailable Fe present in soils.
Shunsuke Watanabe +7 more
wiley +1 more source
Drug-antibody ratio (DAR) of antibody-drug conjugates (ADCs) is important for their therapeutic efficacy and pharmacokinetics, therefore control on DAR in synthesis process is a key for ADC quality control.
Yubo Tang +6 more
doaj +1 more source
Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic to animals and humans and that have putative roles in defense against pests.
Paul Dahlin +4 more
doaj +1 more source
Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance [PDF]
BACKGROUND: β-Glucosidase is an important member of the biomass-degrading enzyme system, and plays vital roles in enzymatic saccharification for biofuels production.
Bin Yao +7 more
core +1 more source
Partial deglycosylation of blood-group-specific glycoproteins [PDF]
The time course for the partial deglycosylation of blood-group-specific glycoproteins from human ovarian-cyst fluids with 0.25 M-H2SO4/acetic acid and 6 M-HCl in methanol was studied. Either reagent readily removed about 80% of the carbohydrate from the glycoproteins to leave non-diffusible glycopeptides that contain N-acetylgalactosamine as the ...
openaire +2 more sources
One‐step, highly efficient, and scalable synthesis of acp3U phosphoramidite is described, as well as a new alternative synthesis of cmnm5U phosphoramidite from a highly versatile intermediate. Latter could be scaled‐up, split, and aliquots turned simultaneously into cmnm5U, nm5U, and mnm5U.
Ewa Mejdr +3 more
wiley +1 more source
PNGase Sensitivity Assay to Study the Folding Status of Proteins
This protocol aims to evaluate folding status of proteins, utilizing peptide:N-glycanase (PNGase) sensitivity. In the cytosol, PNGase works as a deglycosylation-enzyme.
Satoshi Ninagawa, Kazutoshi Mori
doaj +1 more source
The Nicastrin ectodomain adopts a highly thermostable structure [PDF]
Nicastrin is a type I transmembrane glycoprotein, which is part of the high molecular weight gamma-secretase complex. gamma-Secretase is one of the key players associated with the generation of Alzheimer's disease pathology, since it liberates the ...
Beyer, Klaus +6 more
core +2 more sources
The authors employed widely targeted metabolomics, microbial amplicon sequencing, and fermentation assays to investigate the microbiome 's influence on PCRC 's (pericarp of Citrus reticulata ' Chachi ') flavonoid profile over 0–19 years of storage. Based on the correlation analysis, solid‐state and liquid‐state fermentation, and catalytic activity ...
Jianmu Su +10 more
wiley +1 more source

