Results 181 to 190 of about 60,871 (285)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Fermentation quality, microbial dynamics, and rumen degradability of total mixed ration silages containing Manihot sp' foliage. [PDF]

Trop Anim Health Prod
de Sousa AR, de Araújo MJ, Medeiros OT, Dias-Silva TP, Gurgel ALC, de Souza Miranda R, Bezerra LR, Ítavo LCV, Difante GDS, Edvan RL.   +9 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Gene Delivery Mediated by Backbone-Degradable RAFT Copolymers. [PDF]

Biomacromolecules
Mulay PB, Radford DC, Rondon B, Favetta B, Schuster BS, Niu J, Gormley AJ.   +6 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Selecting high-yield forage sorghum genotypes for ensiling: agronomic traits, fermentation parameters, and nutritional value. [PDF]

Sci Rep
da Silva MFP, Rigueira JPS, da Silva PHF, Portugal AF, da Costa Parrella RA, Monção FP, Júnior VRR, Pereira MIB, Araújo IVS, Dos Santos AS.   +9 more
europepmc   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Date palm seed-derived biochar as an environmentally sustainable feed supplement in cattle: impacts on gas production, methane emissions, fermentation parameters and performance predictions. [PDF]

BMC Vet Res
Ghazzawy HS, Alqahtani NK, Sheikh A, El Sayed MS, Mathew RT, Ali-Dinar HM, El-Haroun E, Eissa EH, Abd-Elkarim MM, Abdelnour SA.   +9 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

All-Bamboo Fiber Thermosetting Plastics with Excellent Mechanical Properties, Degradability and High Water Resistance. [PDF]

Polymers (Basel)
Zhang W, Ren W, Liu E, Mou C, Han J, Lv J, Guo D.   +6 more
europepmc   +1 more source