Results 161 to 170 of about 38,202 (297)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Ultralow concentrations of curcumin as a structural modifier in chitosan coatings for eco-friendly postharvest preservation of strawberries. [PDF]

Food Sci Biotechnol
Silva HS, Zanoli APFA, Barbosa HFG, de Oliveira Pedro R.   +3 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Dietary Supplementation of <i>Bacillus subtilis</i> Improves In Vitro Rumen Microbial Fermentation When Using <i>Macadamia integrifolia</i> Husk as a Substrate. [PDF]

Microorganisms
Liu H, Deng X, Chen A, Zeng H, Yang Q, Chen X, Chen K, Huang S, Zhang X, Zhou H, Han J.   +10 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Methane mitigation, nutritional evaluation, and in vitro degradation kinetics of diets based on tropical grass haylage treated with xylanase. [PDF]

Trop Anim Health Prod
Duarte ABA, Dias-Silva TP, Viana WCC, Gurgel ALC, Araújo MJ, Abdalla AL, Bezerra LR, Edvan RL, Nascimento RR, Sousa SV, Marques CAT, Ítavo LCV, Lima PMT.   +12 more
europepmc   +1 more source

Lysosomal degradability of poly(alpha-amino acids)

, 2009
[[abstract]]©1997 Wiley - The lysosomal degradability of poly(alpha-amino acids) based on poly(L-glutamic acid) and its derivatives/copolymers was evaluated to gain insight into the subcellular fate of the macromolecules as water soluble polymeric drug ...
Hsin-Cheng Chiu;Pavla Kopeckova;Sharad S. Deshmane;Jindrich Kopecek
core  

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Polyhydroxyalkanoate/seashell powder biocomposites with degradability and 3D printing applications. [PDF]

Sci Rep
Min W, Guo B, Zhao Z, Hu S, Shang J, Bai Y, Zhang X, Zhong X.   +7 more
europepmc   +1 more source

Functional degradation [PDF]

Nature Immunology, 2019
openaire   +2 more sources