Results 171 to 180 of about 33,224 (307)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Effect of Corn Processing and Protein Degradability on Ruminal Metabolism and Feeding Behavior of Dairy Cows. [PDF]

Animals (Basel)
da Fonseca DCM, Martins CMMR, Alves BG, Fidelis CE, do Santos MV.   +4 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Degradation kinetics and protein intestinal digestibility of tomato pomace in goats. [PDF]

Front Vet Sci
Long Y, Paengkoum S, Lu S, Niu X, Thongpea S, Taethaisong N, Han Y, Paengkoum P.   +7 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Evaluation of Ascophyllum nodosum extract supplementation on feed degradability, ruminal fermentation and methane production using the rumen simulation technique (RUSITEC). [PDF]

PLoS One
Casey A, Boland TM, Kelly AK, McKay ZC, Markiewicz-Keszycka M, Mirzapour-Koudash A, Garcia-Vaquero M, Balakrishnan S, Guinan KJ, O'Sullivan JT, Vigors S.   +10 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Effect of dual bacterial combinations on in vitro nutrient degradability, gas production, methane emission, ruminal fermentation parameters and predictive values in sheep. [PDF]

Sci Rep
Saleem ASA, Bassiony SM, Abdelnour SA, Abdel-Monem UM, Elaref MY, Al-Marakby KM.   +5 more
europepmc   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Effects of Isoacid Supplementation on In Vitro Rumen Fermentation, Nutrient Degradability and Bacterial Community Diversity Using Corn Silage-Highland Barley Straw as Substrates in Yaks. [PDF]

Microorganisms
Luo C, Jiang F, Zhong A, He X, Liu X, Huang Y, Gao Y.   +6 more
europepmc   +1 more source