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Lysophosphatidylserine induces eosinophil extracellular trap formation and degranulation: Implications in severe asthma

Allergy. European Journal of Allergy and Clinical Immunology, 2020
Recent evidence demonstrates that activated eosinophils undergo a distinct form of lytic cell death, accompanied by formation of DNA‐based eosinophil extracellular trap (EET) and degranulation, enhancing inflammatory immune responses in asthmatic airways.
Hye Jeong Kim   +6 more
semanticscholar   +1 more source

Myeloperoxidase Stimulates Neutrophil Degranulation

Bulletin of Experimental Biology and Medicine, 2016
Myeloperoxidase, heme enzyme of azurophilic granules in neutrophils, is released into the extracellular space in the inflammation foci. In neutrophils, it stimulates a dose-dependent release of lactoferrin (a protein of specific granules), lysozyme (a protein of specific and azurophilic granules), and elastase (a protein of azurophilic granules).
D. Grigorieva   +6 more
semanticscholar   +3 more sources

Basophil degranulation control

International Journal of Bio-Medical Computing, 1992
We first present a global simulation model describing inhibition of human basophil degranulation by means of high dilutions. Then we study an optimal control problem associated to a non-linear compartmental model. This control is associated to an antigen concentration. For solving this control problem we used a dynamic programming method.
B, Hadjaj   +2 more
openaire   +2 more sources

Control of basophil degranulation

International Journal of Bio-Medical Computing, 1993
We first present a linear compartmental model describing human basophiol degranulation by means of an antigen concentration. Then we study an optimal control problem associated to this linear compartmental model in presence of high dilutions. For solving this control problem we used the LAPLACE transform.
B, Hadjaj   +2 more
openaire   +2 more sources

Markers of Mast Cell Degranulation

Methods, 1997
Mast cells are the primary effector cells of immediate hypersensitivity reactions in humans. Upon mast cell activation both preformed and newly synthesized mediators are secreted. Histamine can be measured by fluorometric assays, radioenzymatic assays, and immunoassays.
A D, Hogan, L B, Schwartz
openaire   +2 more sources

Basophil Degranulation Assay

2017
Basophil degranulation assay has gained importance over the last decade in both diagnosis of food allergy and evaluation of progression of immunotherapy. This assay involves the identification and quantification of the expression of CD63 molecule on basophil membrane. CD63 is a marker of multivesicular bodies that is exposed to cell membrane during the
Madhan, Masilamani   +2 more
openaire   +2 more sources

Eosinophil degranulation

Inflammation, 1981
A method is described for the quantitative monitoring of human eosinophil degranulation using interference contrast microscopy. Using staphyloccoci as a stimulus, degranulated cells appeared larger than nondegranulating cells, were ameboid in shape and exhibited large nude areas of cytoplasm with prominent nuclei.
R, Sher, A A, Wadee
openaire   +2 more sources

Measuring Degranulation of Mast Cells

Current Protocols in Immunology, 1993
AbstractThis unit describes methods for measuring exocytosis of preformed mediators from secretory granules as an indication of IgE receptor‐mediated activation of mast cells. The first basic protocol describes the measurement of biogenic amines (serotonin and histamine) secreted by activated rodent mast cells (for serotonin) or rodent and human mast ...
R J, Hohman, S C, Dreskin
openaire   +2 more sources

Degranulation of Human Platelets by the Thrombin Receptor Peptide SFLLRN: Comparison with Degranulation by Thrombin

Thrombosis and Haemostasis, 1993
SummaryA new, simplified method of degranulating human platelets using the thrombin receptor peptide SFLLRN (20 |iM) is described; released fibrinogen cannot be converted to fibrin, and the platelets are not exposed to a proteolytic enzyme, as they are when thrombin is used for degranulation.
R L, Kinlough-Rathbone   +4 more
openaire   +2 more sources

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