Results 71 to 80 of about 388,663 (309)
Secondary structures and their effects on antioxidant capacity of antioxidant peptides in yogurt
International Journal of Food Properties, 2018 The pH, thermal, urea, and trifluoroethanol treatments were used to denature yogurt antioxidant peptides, and their antioxidant capacities and secondary structures were analysed.
HaiNa Yuan +6 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Chaperonin GroEL Reassembly: An Effect of Protein Ligands and Solvent Composition
Biomolecules, 2014 Chaperonin GroEL is a complex oligomeric heat shock protein (Hsp60) assisting the correct folding and assembly of other proteins in the cell. An intriguing question is how GroEL folds itself.
Nataliya Ryabova +3 more
doaj +1 more source
Local denaturation of DNA by shearing forces and by heat
, 1963 The DNA of phage T5 is partially denatured by stirring at 25°C in 0·1 M- or 0·6 M-NaCl at speeds and DNA concentrations similar to those required to initiate breakage of the molecules.
Burgi, E. +7 more
core +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
THE DENATURATION OF FISH MUSCLE PROTEINS BY FREEZING
, 1933 The rate and extent of protein denaturation in frozen fish muscle juice have been determined. The extent of denaturation is greater at −2 °C., approximately 25 per cent, of the total protein being precipitated in 84 days.
D. B. FINN
core +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Journal of the Turkish Chemical Society, Section A: Chemistry, 2017 The origins of the Hydrophobic Effect (HE), its biological significance and its experimental basis are critically addressed in this brief review. It is argued that the mechanistic work reported on the HE in recent decades needs to be reassessed, as its ...
Sosale Chandrasekhar
doaj +1 more source
FEBS Open Bio, EarlyView.A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li +7 more
wiley +1 more source
Scientia Agricola, 2006 Hen eggwhite proteins have been extensively utilized as ingredients in food processing because of their unique functional properties, such as gelling and foaming. This work reviews the molecular basis of the eggwhite proteins targeting the development of
Ana Cláudia Carraro Alleoni
doaj +1 more source