Results 31 to 40 of about 2,203,280 (253)

Biophysical characterization of the structural stability of Helix lucorum hemocyanin

Biotechnology & Biotechnological Equipment, 2021
The structural stability of the hеmocyanin purified from the hеmolymph of gаrden snаils Helix lucorum (HlH) was investigated by means of far-UV circular dichroism (CD), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM ...
Krassimira Idakieva, Svetla Todinova, Aleksandar Dolashki, Lyudmila Velkova, Yuliana Raynova, Pavlina Dolashka   +5 more
doaj   +1 more source

Smaller is better: nanobodies meet NMR

FEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

Influence of Ficoll on urea induced denaturation of fibrinogen

AIP Advances, 2016
Ficoll is a neutral, highly branched polymer used as a molecular crowder in the study of proteins. Ficoll is also part of Ficoll-Paque used in biology laboratories to separate blood to its components (erythrocytes, leukocytes etc.,).
Kamatchi Sankaranarayanan, N. Meenakshisundaram   +1 more
doaj   +1 more source

Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: Denaturation/unfolding coupled hydrolysis mechanism

Current Research in Food Science, 2022
The inactivation of antinutritional factors, protease inhibitors within winged bean protein was induced by two respective method treatments. The physical method based on steam vapor that was conducted using an autoclave and chemical method consisting on ...
Sami Saadi, Nazamid Saari, Hasanah Mohd Ghazali, Mohammed Sabo Abdulkarim   +3 more
doaj   +1 more source

Decoupling enzyme catalysis from thermal denaturation [PDF]

, 2010
The equilibrium model (EM) (Daniel et al., 2001) postulates two forms of a folded enzyme, one catalytically active (Eact) and the other inactive (Einact), which interconvert via a fast thermal equilibrium (Keq) (Figure A). This model for enzyme catalysis
Easter, Ashley Davys
core  

The Alkaline Denaturation of DNA [PDF]

, 1969
A kinetic study of the alkaline transition of DNA, in clearly defined physico-chemical conditions, is presented, which allows us to identify, within the alkaline transition region, different pH ranges, corresponding to different ratelimiting factors ...
Ageno, M., Frontali, C., Dore, E.
core   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Entropy-driven denaturation enables sustainable protein regeneration through rapid gel-solid transition

Nature Communications
The upcycling of protein materials has long been hindered by the difficulty in restructuring them to usable forms. In contrast to proteins extracted using conventional organic denaturants, keratin treated with concentrated inorganic lithium bromide (LiBr)
Yichong Wang, Junlang Liu, Michael M. Peters, Ryoma Ishii, Dianzhuo Wang, Sourav Chowdhury, Kevin Kit Parker, Eugene I. Shakhnovich   +7 more
doaj   +1 more source