Results 41 to 50 of about 33,616 (245)

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

Stochastic Nanoscale Biophysical Cues as a Basis for the Induction of Glioblastoma‐Like Transcriptional Programs in Astrocytes

Advanced Science, EarlyView.
Stochastic nanoscale physical cues induce glioblastoma (GBM)‐associated transcriptional traits in naïve astrocytes leading to spontaneous formation of spheroids. Cells within spheroids express activated‐MMP2 and a differential gene expression pattern involving P53 and NOTCH3, providing evidence for a role for changes in brain topography, as observed in
Laurent Starck, Tobias Butelmann, Sabrina Hogan, Melika Sarem, Bernd Heimrich, Ritwick Sawarkar, Marie‐Françoise Ritz, Gregor Hutter, V. Prasad Shastri   +8 more
wiley   +1 more source

Changes in proteolytic bacteria in paddy soils in response to organic management

Acta Agriculturae Scandinavica. Section B, Soil and Plant Science, 2017
Proteolytic bacterial communities, which mineralize organic nitrogen, play a key role in agricultural systems. In this study, alkaline metalloprotease (apr) gene fragments from proteolytic bacteria were investigated in bulk and rhizosphere paddy soil ...
Shu Wang, Xiping Cheng
doaj   +1 more source

Unraveling the Morphological and Functional Maturation Mechanisms Underlying Human Neural Development Using iPSCs‐Derived Neuronal Model

Advanced Science, EarlyView.
Using human induced pluripotent stem cells (hiPSCs)‐derived neuronal model, Tian and colleagues reveal that voltage‐gated calcium channels Cav1.2 and Cav1.3, and their mediated calcium ion influx, are essential for early morphogenesis of human neuronal development, while ECEL1 underlies human neuronal functional developmental maturation through CALM3 ...
Yue Tian, Yi‐Chun Ou, Zi‐Xian Zhang, Jie Cai, Si‐Qing Cai, Guo‐Gang Xing   +5 more
wiley   +1 more source

Denaturing gradient gel electrophoresis as a diagnostic tool in periodontal microbiology. [PDF]

J Clin Microbiol, 2006
ABSTRACT Bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. The aim of the present study was to show the potential of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant ...
Zijnge V, Welling GW, Degener JE, van Winkelhoff AJ, Abbas F, Harmsen HJ.   +5 more
europepmc   +7 more sources

Detecting prion protein gene mutations by denaturing gradient gel electrophoresis [PDF]

Human Mutation, 1994
Mutations of the prion protein (PrP) gene are present in patients with Gerstmann-Sträussler-Scheinker syndrome (GSS), familial Creutzfeldt-Jakob disease (CJD), and fatal familial insomnia (FFI). We developed a denaturing gradient gel electrophoresis (DGGE) strategy that readily identifies point mutations in the PrP coding sequence.
Fink, John K., Peacock, Michael L., Warren, James T., Roses, Allen D., Prusiner, Stanley B.   +4 more
openaire   +3 more sources

Fibrates Inhibit PLTP‐induced M2 Macrophage Infiltration and Increase the Sensitivity of Hepatocellular Carcinoma to ICIs

Advanced Science, EarlyView.
Phospholipid transfer protein(PLTP) plays a critical role in forming a complex with kinase A (AURKA) and P65. This interaction facilitates phosphorylation of P65 at Ser536, leading to the activation of the NF‐κB signaling pathway. Ultimately, this leads to the upregulation of downstream cytokines, including IL‐6, IL‐8, and CSF‐1, which promotes M2 ...
Xinyue Liang, Yaning Li, Jianxun Cai, Lisi Luo, Pengfei Yang, Yutong Chen, Jiancong Zhou, Yan Zeng, Jiaping Yu, Weiyu Zhang, Wenzheng Pang, Yufang Li, Chunhua Wen, Jian Li, Linjuan Zeng   +14 more
wiley   +1 more source

Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase from human liver.

Journal of Lipid Research, 1982
The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase from human liver was purified to homogeneity. Electrophoresis of the purified human enzyme on non-denaturing gels revealed a single protein-staining band that co-migrated with reductase activity ...
R D Tanaka, P A Edwards, S F Lan, E M Knöppel, A M Fogelman   +4 more
doaj   +1 more source

MicroRNA‐375‐3p Targets Fatty Acid Synthase and Relish to Regulate Energy Allocation During Pupal Metamorphosis and Starvation

Advanced Science, EarlyView.
During pupal metamorphosis and starvation, elevated 20‐hydroxyecdysone (20E) and suppressed insulin trigger Forkhead box O (FOXO) nuclear translocation, enhancing miR‐375‐3p expression. This downregulates fatty acid synthase (FASN) and Relish, promoting lipid breakdown for energy while prioritizing antioxidant responses over immune functions to support
Peng Chen, Meiqi Cheng, Jianhui Wang, Jing Tang, Xiaoqiao Huang, Yusi Li, Huiling Zhou, Ling Zhang, Yi Dong, Chengjun Li, Bin Li   +10 more
wiley   +1 more source

Ufmylation‐Deficient DDRGK1 Ameliorates Obesity by Inhibiting FASN‐Mediated Adipocyte Lipogenesis

Advanced Science, EarlyView.
DDRGK1 regulates de novo lipogenesis via stabilization of fatty acid synthase (FASN). DDRGK1‐mediated UFMylation of FASN prevents its ubiquitin–proteasomal degradation. Reduced DDRGK1 expression or mutation at the key UFMylation site enhances FASN degradation and suppresses fatty acid synthesis (FAS), resulting in smaller adipocytes and improved ...
Yin Li, Tangjun Zhou, Xiao Yang, Kewei Rong, Xiankun Cao, Lei Shi, Xin Wang, Hongjin Wan, Lei Cui, Kexin Liu, Tong Xing, Hang Zhang, Chen Zhao, Tingxian Guo, Peixiang Ma, Jie Zhao, An Qin   +16 more
wiley   +1 more source