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Density gradient centrifugations of adenovirus-antibody complexes
Archiv f�r die gesamte Virusforschung, 1965Buoyant density sedimentations in CsCl of adenovirus type 5 and virus-antibody complexes has been described. It has been demonstrated that virus which survives a certain dose of antibody is found at densities lower than that of untreated particles suggesting a coating of the virus with antibody.
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Centrifugation of brain microsomes in a density gradient
Experimental Cell Research, 1960Abstract Microsomal preparations from rat brain were centrifuged in various density gradients obtained with different concentrations of sucrose. The different zones of the gradients were analyzed chemically as to their protein and RNA content and examined with the electron microscope as to their content of particles and membrane structures.
V, HANZON, G, TOSCHI
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Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation
2023Sophisticated organelle fractionation strategies were the workhorse of early peroxisome research and led to the characterization of the principal functions of the organelle. However, even in the era of molecular biology and "omics" technologies, they are still of importance to unravel peroxisome-specific proteomes, confirm the localization of still ...
Andreas, Manner, Markus, Islinger
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Centrifugation of Liver Microsomes on Metrizamide Density Gradients
Preparative Biochemistry, 1976Abstract Metrizamide, 2-(3-acetamido-5-N-methyl-acetamido-2, 4, 6–triiodobenzamido)-2-deoxy-D-glucose (mol. wt 789), inhibits liver microsomal enzymes only to a small degree and it has no solubilization or detergent effects on the membrane. Four hour centrifugation in a continuous metrizamide gradient is sufficient for microsomal vesicles to attain ...
H, Fehrnström +2 more
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The Effects of Overloading in Density-Gradient Centrifugation
European Journal of Biochemistry, 1975The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable ...
J, Steensgaard, N P, Moller, L, Funding
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Lymphocyte Populations: Separation by Discontinuous Density Gradient Centrifugation
The Journal of Immunology, 1973Abstract A method for separation of human lymphocytes by discontinuous density gradient is described. The method was simple to carry out and utilized Ficoll-salt solutions which were easily standardized. There was good reproducibility with no toxicity to the cells.
D T, Yu +3 more
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Isolation of bovine eosinophils by density gradient centrifugation
American Journal of Veterinary Research, 1985SUMMARY A method for the isolation of an enriched population (> 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092.
W H, Chambers +3 more
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Isolation of Endocitic Organelles by Density Gradient Centrifugation
2008Advanced prefractionation strategies, in combination with highly sensitive and accurate mass spectrometers provide powerful means to detect and analyze low abundant proteins on the subcellular and organelle-specific level. Among enrichment techniques, subcellular fractionation has become the most commonly used.
Mariana Eça Guimarães, de Araùjo +2 more
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Density gradient centrifugation studies of vesicular stomatitis virus
Archiv f�r die gesamte Virusforschung, 1965Equilibrium sedimentation in caesium chloride has been used to separate and study the biologically active components of vesicular stomatitis virus (Indiana) grown in baby hamster kidney cells and chick embryos. Infective egg fluid was shown to contain two infective components of densities 1.191 g/ml. and 1.217 g/ml.
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An improved gradient-making device for density gradient centrifugation
Analytical Biochemistry, 1965Abstract An improved gradient-making device for sucrose density gradient centrifugation is described. The device can be easily constructed with a minimum of glassblowing skill. An alternate mode of operation is presented which allows the production of a linear sucrose density gradient even from a poorly constructed device.
T. Salo, D.M. Kouns
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