Results 251 to 260 of about 226,785 (298)
Some of the next articles are maybe not open access.
Centrifugation of Liver Microsomes on Metrizamide Density Gradients
Preparative Biochemistry, 1976Abstract Metrizamide, 2-(3-acetamido-5-N-methyl-acetamido-2, 4, 6–triiodobenzamido)-2-deoxy-D-glucose (mol. wt 789), inhibits liver microsomal enzymes only to a small degree and it has no solubilization or detergent effects on the membrane. Four hour centrifugation in a continuous metrizamide gradient is sufficient for microsomal vesicles to attain ...
H, Fehrnström +2 more
openaire +2 more sources
The Effects of Overloading in Density-Gradient Centrifugation
European Journal of Biochemistry, 1975The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable ...
J, Steensgaard, N P, Moller, L, Funding
openaire +2 more sources
Lymphocyte Populations: Separation by Discontinuous Density Gradient Centrifugation
The Journal of Immunology, 1973Abstract A method for separation of human lymphocytes by discontinuous density gradient is described. The method was simple to carry out and utilized Ficoll-salt solutions which were easily standardized. There was good reproducibility with no toxicity to the cells.
D T, Yu +3 more
openaire +2 more sources
Isolation of bovine eosinophils by density gradient centrifugation
American Journal of Veterinary Research, 1985SUMMARY A method for the isolation of an enriched population (> 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092.
W H, Chambers +3 more
openaire +2 more sources
Isolation of Endocitic Organelles by Density Gradient Centrifugation
2008Advanced prefractionation strategies, in combination with highly sensitive and accurate mass spectrometers provide powerful means to detect and analyze low abundant proteins on the subcellular and organelle-specific level. Among enrichment techniques, subcellular fractionation has become the most commonly used.
Mariana Eça Guimarães, de Araùjo +2 more
openaire +2 more sources
Density gradient centrifugation studies of vesicular stomatitis virus
Archiv f�r die gesamte Virusforschung, 1965Equilibrium sedimentation in caesium chloride has been used to separate and study the biologically active components of vesicular stomatitis virus (Indiana) grown in baby hamster kidney cells and chick embryos. Infective egg fluid was shown to contain two infective components of densities 1.191 g/ml. and 1.217 g/ml.
openaire +2 more sources
An improved gradient-making device for density gradient centrifugation
Analytical Biochemistry, 1965Abstract An improved gradient-making device for sucrose density gradient centrifugation is described. The device can be easily constructed with a minimum of glassblowing skill. An alternate mode of operation is presented which allows the production of a linear sucrose density gradient even from a poorly constructed device.
T. Salo, D.M. Kouns
openaire +1 more source
Preparation of polyallomer centrifuge tubes for density gradients
Analytical Biochemistry, 1969Abstract Thin-walled polyallomer centrifuge tubes can easily be prepared to accept linear sucrose gradients, as delivered by conventional gradient formers. They can thus be used with the same facility and achieve the same resolution as cellulose nitrate tubes, for preparative and analytical density gradient centrifugation.
openaire +2 more sources
Rapid density gradient centrifugation using Short Column Techniques
Analytical Biochemistry, 1978Abstract The Short Column Technique is a useful technique for rate zonal and isopycnic density gradient runs with both the swinging bucket and fixed angle rotors, We have described and illustrated its advantages of significant time saving and the elimination of the need for tube caps. Thick-walled polycarbonate tubes are used.
openaire +2 more sources