Results 261 to 270 of about 39,457 (305)

A possible model for the methylation of deoxycytidine in DNA

Medical Hypotheses, 1984
The modified base 5-methylcytidine has been found in the DNA of a number of different eukaryotic cells where it occurs principally in the dinucleotide sequence -CmpG- which is present as a palindrome in double-strand nucleic acid molecules. There is considerable evidence to indicate and suggest that 5-methylcytosine serves as a regulatory signal in ...
A O, Hawtrey, M, Ariatti
openaire   +2 more sources

Development of enzyme immunoassay of 2′-deoxycytidine

Journal of Pharmaceutical and Biomedical Analysis, 1997
In order to study 2'-deoxycytidine (2'-dCyd) as a possible prognostic marker in cancer chemotherapy, an enzyme immunoassay (EIA) was developed. 2'-dCyd as a hapten was succinylated and two omicron-monosuccinyl-2'-dCyd's were purified by high performance liquid chromatography and identified by mass spectrometry and 1H-NMR.
K, Omura, K, Hirose, M, Itoh, T, Akizawa, M, Yoshioka   +4 more
openaire   +2 more sources

Regulation of human deoxycytidine kinase expression

Advances in Enzyme Regulation, 1993
The human deoxycytidine kinase gene is a single copy gene and is comprised of seven exons that are spread over more than 34 kb of the genome. The 5'-flanking region is highly G/C rich and does not contain CAAT or TATA boxes. This region, when cloned into a recorder gene construct containing the chloramphenicol acetyltransferase gene, is capable of ...
B S, Mitchell, J J, Song, E E, Johnson, E, Chen, J S, Dayton   +4 more
openaire   +2 more sources

Electron Attachment to Microhydrated Deoxycytidine Monophosphate

The Journal of Physical Chemistry B, 2018
DNA constituents are effectively decomposed via dissociative electron attachment (DEA). However, the DEA contribution to radiation damage in living tissues is a subject of ongoing discussion. We address an essential question, how aqueous environment influences the DEA to DNA.
Jaroslav Kočišek, Barbora Sedmidubská, Suvasthika Indrajith, Michal Fárník, Juraj Fedor   +4 more
openaire   +3 more sources

Effect of Phosphorylation on Deoxycytidine Kinase Activity

2006
In contrast to deoxycytidine kinase from leukemic blasts7, the recombinant dCK is a poor substrate for protein kinase C. Protein kinase A effectively phosphorylates the recombinant dCK. However, neither substrate specificity, nor kinetic parameters of dCK were changed upon phosphorylation.
T, Spasokoukotskaja, Z, Csapo, M, Sasvari-Szekely, S, Virga, I, Talianidis, S, Eriksson, M, Staub   +6 more
openaire   +2 more sources

Human Deoxycytidine Kinase as a Deoxyribonucleoside Phosphorylase

Journal of Molecular Biology, 2004
Human deoxycytidine kinase (dCK) is a key enzyme in the 5'-phosphorylation of purine and pyrimidine deoxynucleosides with deoxycytidine as the most efficient substrate. The ability of dCK to degrade 2'-deoxyribonucleosides to free nucleobases and 2-deoxy-alpha-d-ribofuranose-1-phosphate was demonstrated by 1H-31P correlation spectroscopy and by isotope
Elena, Usova, Tatiana, Maltseva, András, Földesi, Jyoti, Chattopadhayaya, Staffan, Eriksson   +4 more
openaire   +2 more sources

Metabolism of deoxycytidine in isolated perfused organs

Biophysik, 1972
The metabolism of deoxycytidine-U-14C was studied in isolated perfused normal and regenerating rat liver, in perfused rat intestine and in isolated perfused mouse liver. A technique to perfuse mouse liver is described. Catabolism of dCyd is very slow in organs of the rat whereas it is rapid in those of the mouse.
G B, Gerber, B, Zicha, J, Deroo, J P, De Cock, E, Geyer   +4 more
openaire   +2 more sources

5'-Substituted 2'-Deoxycytidines as Non-Substrate Inhibitors of Human Deoxycytidine Kinase

Nucleosides, Nucleotides and Nucleic Acids, 1995
Abstract Several 5′-substituted analogues of 2′-deoxycytidine (dC), including 5′-amino2′,5′-dideoxycytidine (5′-amino-ddC), 5′-azido-2′,5′-dideoxycytidine (5′-azido-ddC) and 5′-O-ethyl-2′-deoxycytidine (5′-O-ethyl-dC), are non-substrate inhibitors of human dC kinase.
K. Krawiec, B. Kierdaszuk, D. Shugar
openaire   +1 more source

2'-Deoxycytidine-N3-cyanoborane

Acta Crystallographica Section C Crystal Structure Communications, 1996
The structure of the P2 1 2 1 2 1 form of 2'-deoxycytidineN3-cyanoborane, C 10 H 15 BN 4 O 4 , has been determined. The sugar is in the 2 E puckering mode and the C5'-05' bond has a tg conformation while the relative orientation of the sugar and the base remains anti.
Q. Gao, A. Sood, B. Ramsay Shaw, L. D. Williams   +3 more
openaire   +1 more source

Structure of 5-methyl-2'-deoxycytidine

Acta Crystallographica Section C Crystal Structure Communications, 1988
C10H15N3O4, Mr = 241.2, orthorhombic, P2(1)2(1)2(1), a = 10.454 (1), b = 11.922 (1), c = 9.057 (1) A, V = 1128.9 (1) A3, Z = 4, D chi = 1.419 Mg m-3, Cu K alpha radiation, lambda = 1.54178 A, mu = 0.95 mm-1, F(000) = 512, room temperature, R = 0.029 for 1186 observed reflections.
openaire   +2 more sources