Results 41 to 50 of about 39,457 (305)

Synthesis of Oligonucleotides Containing Two Putatively Mutagenic DNA Lesions:  5-Hydroxy-2‘-deoxyuridine and 5-Hydroxy-2‘-deoxycytidine

, 2016
Spontaneous oxidative DNA damage occurs as a consequence of aerobic metabolism, lipid peroxidation, immune responses, ionizing radiation, and some chemical oxidants. These processes yield a vast array of oxidized DNA bases and sugars.
Deborah A. Kreutzer (3088191), Marshall L. Morningstar (3088188), John M. Essigmann (357654)   +2 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Long-term safety and efficacy of deoxycytidine/deoxythymidine in treatment of POLG-related disorders

Neurotherapeutics
We evaluated the safety and efficacy of enteral deoxycytidine/deoxythymidine combination therapy in treatment of POLG-related disorders, genetic mitochondrial diseases characterized by progressive neurological degeneration.
Anthony C.T. Cheung, Saoussen Berrahmoune, Christelle Dassi, Heather Pekeles, Tommy Gagnon, Paula J. Waters, Ralf Eberhard, Daniela Buhas, Kenneth A. Myers   +8 more
doaj   +1 more source

Epigenetic factors in cancer risk: effect of chemical carcinogens on global DNA methylation pattern in human TK6 cells.

PLoS ONE, 2012
In the current study, we assessed the global DNA methylation changes in human lymphoblastoid (TK6) cells in vitro in response to 5 direct and 10 indirect-acting genotoxic agents.
Ali M Tabish, Katrien Poels, Peter Hoet, Lode Godderis   +3 more
doaj   +1 more source

Deoxycytidine and Deoxythymidine Treatment for Thymidine Kinase 2 Deficiency [PDF]

, 2017
Objective: Thymidine kinase 2 (TK2), a critical enzyme in the mitochondrial pyrimidine salvage pathway, is essential for mitochondrial DNA (mtDNA) maintenance.
Garone C., Tadesse S., Sanchez-Quintero M. J., Hirano M., Levy R. J., Barca E., Lopez-Gomez C., Garcia-Diaz B., Juanola-Falgarona M.   +8 more
core   +1 more source

Azaporphyrinoid‐Based Photo‐ and Electroactive Architectures for Advanced Functional Materials

Advanced Materials, EarlyView.
A long‐standing collaboration between the Torres and Guldi groups has yielded diverse azaporphyrinoid‐based donor‐acceptor nanohybrids with promising applications in solar energy conversion. This conspectus highlights key molecular platforms and structure‐function relationships that govern light and charge management, supporting the rational design of ...
Jorge Labella, Kobra Azizi, Dirk M. Guldi, Tomás Torres   +3 more
wiley   +1 more source

Global DNA Methylation in Cord Blood as a Biomarker for Prenatal Lead and Antimony Exposures

Toxics, 2022
DNA methylation is an epigenetic mechanism for gene expression modulation and can be used as a predictor of future disease risks. A prospective birth cohort study was performed to clarify the effects of neurotoxicants on child development, namely, the ...
Yoshinori Okamoto, Miyuki Iwai-Shimada, Kunihiko Nakai, Nozomi Tatsuta, Yoko Mori, Akira Aoki, Nakao Kojima, Tatsuyuki Takada, Hiroshi Satoh, Hideto Jinno   +9 more
doaj   +1 more source

Unraveling the Molecular Mechanisms Underlying Spontaneous Multipolar Mitosis Through CIN‐seq

Advanced Science, EarlyView.
Multipolar mitosis, a hallmark of chromosomal instability (CIN), drives tumor heterogeneity but is challenging to study in live cells. Using CIN‐seq, a single‐cell multiomics method, we profiled rare CIN events and identified mechanisms associated with viable multipolar mitosis, including PTEN attenuation, Rho GTPase‐driven cytokinesis failure, and ...
Pin‐Rui Su, Ting‐Chun Chou, Maria Teresa López‐Cascales, Junfeng Huang, Tsai‐Ying Chen, Sjoukje van Wieren, Chan Li, Cecile Beerens, Li You, Jelle Storteboom, Miao‐Ping Chien   +10 more
wiley   +1 more source

FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine

Cell Death and Disease, 2022
Fanconi anemia (FA) is a rare hereditary disorder caused by mutations in any one of the FANC genes. FA cells are mainly characterized by extreme hypersensitivity to interstrand crosslink (ICL) agents.
María José Peña-Gómez, Paula Moreno-Gordillo, Milda Narmontė, Clara B. García-Calderón, Audronė Rukšėnaitė, Saulius Klimašauskas, Iván V. Rosado   +6 more
doaj   +1 more source

Improved synthesis of 5-hydroxymethyl-2'-deoxycytidine phosphoramidite using a 2'-deoxyuridine to 2'-deoxycytidine conversion without temporary protecting groups.

, 2011
5-Hydroxymethylcytosine has recently been characterized as the 'sixth base' in human DNA. To enable research on this DNA modification, we report an improved method for the synthesis of 5-hydroxymethyl-2'-deoxycytidine ((5-HOMe)dC) phosphoramidite for ...
Hansen, AS, El-Sagheer, AH, Brown, T, Brown, Tom, Schofield, CJ, Schofield, Christopher, Thalhammer, A   +6 more
core   +1 more source