Results 181 to 190 of about 25,107 (224)

Human T-lymphoblast deoxycytidine kinase: purification and properties

Biochemistry, 1989
Previous observations present tremendous variations in the properties of deoxycytidine kinase. To clarify the properties and physiologic role of deoxycytidine kinase, we have undertaken its purification. Deoxycytidine kinase was purified from cultured human T-lymphoblasts (MOLT-4) to 90% purity with an estimated specific activity of 8 mumol min-1 (mg ...
N S, Datta, D S, Shewach, M C, Hurley, B S, Mitchell, I H, Fox   +4 more
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Effect of Phosphorylation on Deoxycytidine Kinase Activity

2006
In contrast to deoxycytidine kinase from leukemic blasts7, the recombinant dCK is a poor substrate for protein kinase C. Protein kinase A effectively phosphorylates the recombinant dCK. However, neither substrate specificity, nor kinetic parameters of dCK were changed upon phosphorylation.
T, Spasokoukotskaja, Z, Csapo, M, Sasvari-Szekely, S, Virga, I, Talianidis, S, Eriksson, M, Staub   +6 more
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5-Fluorocytosine derivatives as inhibitors of deoxycytidine kinase

Bioorganic & Medicinal Chemistry Letters, 2009
A series of potent piperidine-linked cytosine derivatives were prepared as inhibitors of deoxycytidine kinase (dCK). Compound 9h was discovered to be a potent inhibitor of dCK and shows a good combination of cellular potency and pharmacokinetic parameters.
James E, Tarver, Theodore C, Jessop, Marianne, Carlsen, David J, Augeri, Qinghong, Fu, Jason P, Healy, Alexander, Heim-Riether, Amy, Xu, Jerry A, Taylor, Min, Shen, Philip E, Keyes, S, David Kimball, Xuan-Chuan, Yu, Maricar, Miranda, Qingyun, Liu, Jonathan C, Swaffield, Amr, Nouraldeen, Alan G E, Wilson, Rick, Finch, Kanchan, Jhaver, Ann Marie Digeorge, Foushee, Steve, Anderson, Tamas, Oravecz, Kenneth G, Carson   +23 more
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5'-Substituted 2'-Deoxycytidines as Non-Substrate Inhibitors of Human Deoxycytidine Kinase

Nucleosides, Nucleotides and Nucleic Acids, 1995
Abstract Several 5′-substituted analogues of 2′-deoxycytidine (dC), including 5′-amino2′,5′-dideoxycytidine (5′-amino-ddC), 5′-azido-2′,5′-dideoxycytidine (5′-azido-ddC) and 5′-O-ethyl-2′-deoxycytidine (5′-O-ethyl-dC), are non-substrate inhibitors of human dC kinase.
K. Krawiec, B. Kierdaszuk, D. Shugar
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[43] Deoxycytidine kinase from calf thymus

1978
Publisher Summary This chapter describes the purification procedure of deoxycytidine kinase enzyme from calf thymus. This enzyme from the cytosol of calf thymus is so named because deoxycytidine (dCyd) is the preferred substrate at low concentrations, although its specificity is rather broad.
David H. Ives, Sue-May Wang
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Activation of Deoxycytidine Kinase by Various Nucleoside Analogues

1998
The effect of different nucleoside analogues on deoxycytidine kinase (dCK) and thymidine kinase (TK) was compared in normal human lymphocytes and various leukemic cell lines. G-phase enriched tonsilar lymphocyte subpopulation treated by CdA showed more profound stimulation of dCK activity than S-phase cells.
T, Spasokoukotskaja, M, Sasvári-Székely, L, Hullán, F, Albertioni, S, Eriksson, M, Staub   +5 more
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Specific selection of deoxycytidine kinase mutants with tritiated deoxyadenosine

Biochemical Genetics, 1995
We have shown previously that a low concentration of tritiated deoxyadenosine, i.e., 1 microCi/ml, selectively kills wild-type S49 murine lymphoma cells. Mutant cells resistant to [3H] deoxyadenosine lacked adenosine kinase completely but retained a significant level of deoxyadenosine phosphorylating activity.
T S, Chan, J A, Nelson
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Thymidine kinase and deoxycytidine kinase in HIV-infected children.

The new microbiologica, 2009
It has been demonstrated that HIV infection may affect the levels of thymidine kinase (TK) and deoxycytidine kinase (dCK) in peripheral blood mononuclear cells from HIV infected adults. The aim of this study was to examine the effect of HIV infection and/or antiretroviral therapy on the activity of the above enzymes in HIV-infected children.
FALASCA, FRANCESCA, MAIDA P, BONI A, BERNARDI S, CURSI L, RIZZO B, ANTONELLI, Guido, GATTINARA GC, TURRIZIANI, Ombretta   +8 more
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Deoxycytidine kinase mRNA expression in childhood acute lymphoblastic leukemia

Anti-Cancer Drugs, 1997
Initial and relapsed childhood acute lymphoblastic leukemias (ALL) were investigated for mutations and expression of deoxycytidine kinase (dCK). dCK mediating toxicity of 1-beta-D-arabinofuranosylcytosine was analyzed semiquantitatively by RT-PCR and by single-strand conformation polymorphism.
G, Stammler, F, Zintl, A, Sauerbrey, M, Volm   +3 more
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Cloning of the Dck gene encoding rat deoxycytidine kinase

Gene, 1994
In order to study the mutational inactivation of deoxycytidine kinase (Dck) in a rat model for acute myeloid leukemia we have cloned the complete coding region of the rat Dck gene. Using primers chosen from the human Dck cDNA sequence, we obtained a rat-specific probe via PCR and used it to isolate two clones from a rat lymphocyte cDNA library. The ORF
A P, Stegmann, M W, Honders, R, Willemze, J E, Landegent   +3 more
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