Results 181 to 190 of about 14,183,395 (235)

Periodicity of Deoxyribonuclease I Digestion of Chromatin

Science, 1979
Two methods have been used to measure the single-strand lengths of the DNA fragments produced by deoxyribonuclease I digestion of chromatin. The average lengths obtained are multiples of about 10.4 bases, significantly different from the value of 10 previously reported.
Aaron Klug, Roger D. Kornberg, Francis Crick, Michael Levitt, Ariel Prunell, L. C. Lutter   +5 more
openaire   +4 more sources

Comparative biochemical properties of vertebrate deoxyribonuclease I

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2012
Deoxyribonuclease I (DNase I, EC 3.1.21.1) is an endonuclease that preferentially attacks double-stranded DNA in a Ca(2+)-dependent manner to produce oligonucleotides with 5'-phospho and 3'-hydroxy termini. This review deals with the biochemical properties and molecular evolution of DNase I. A comparative study of vertebrate DNase I from Chondrichthyes
Reiko Iida, Haruo Takeshita, Junko Fujihara, Toshihiro Yasuda, Misuzu Ueki   +4 more
openaire   +4 more sources

Repurposing of 8‐Hydroxyquinoline‐Based Butyrylcholinesterase and Cathepsin B Ligands as Potent Nonpeptidic Deoxyribonuclease I Inhibitors

ChemMedChem, 2022
A library of 31 butyrylcholinesterase (BChE) and cathepsin B (CatB) inhibitors was screened in vitro for inhibition of deoxyribonuclease I (DNase I). Compounds 22, 8 and 7 are among the most potent synthetic non‐peptide DNase I inhibitors reported to ...
Mihajlo Gajić, D. Knez, Izidor Sosič, Janez Mravljak, A. Meden, Urban Košak, Luisa Leitzbach, S. George, B. Hofmann, A. Živković, D. Steinhilber, H. Stark, S. Gobec, A. Šmelcerović, M. Anderluh   +14 more
semanticscholar   +1 more source

1,2,3,4‐Tetrahydroisoquinoline Derivatives as a Novel Deoxyribonuclease I Inhibitors

Chemistry and Biodiversity, 2021
Herein we report an assessment of 24 1,2,3,4‐tetrahydroisoquinoline derivatives for potential DNase I (deoxyribonuclease I) inhibitory properties in vitro. Four of them inhibited DNase I with IC50 values below 200 μM. The most potent was 1‐(6,7‐dimethoxy‐
Mihajlo Gajić, B. Ilić, B. Bondžić, Z. Džambaski, V. Kojić, D. Jakimov, G. Kocic, A. Šmelcerović   +7 more
semanticscholar   +1 more source

PEGylation of Recombinant Human Deoxyribonuclease I Provides a Long‐Acting Version of the Mucolytic for Patients with Cystic Fibrosis

, 2020
Inhalation of recombinant human deoxyribonuclease I (rhDNase) is a gold‐standard therapy in the management of cystic fibrosis (CF). Yet, the rapid elimination of the mucolytic from the lungs requires its daily administration and rhDNase contributes to ...
Marie-Julie Guichard, T. Wilms, Sohaib Mahri, Harshad P. Patil, D. Hoton, B. Ucakar, K. Vanvarenberg, Paméla Cheou, M. Beka, E. Marbaix, T. Leal, R. Vanbever   +11 more
semanticscholar   +1 more source

Active Site of Deoxyribonuclease I: I. The Nature of Active Site of Deoxyribonuclease I

Radiation Research, 1961
Ionizing radiation and specific inhibitors for amino acid residues were used to demonstrate which amino acid residues are involved in the active site of the enzyme deoxyribonuclease I. Bromoacetic acid, lodoacetic acid, diisopropylfluorophosphate, p-chloromercuribenozate, tryptophan, histidine, cinnamic alcohol, cinnamaldehyde, indole, arginine, and ...
S. Okada, G. L. Fletcher
openaire   +3 more sources

Development of a radioimmunoassay for human deoxyribonuclease I

Clinica Chimica Acta, 1986
A reliable radioimmunoassay (RIA) for human deoxyribonuclease I (DNase I) is described. Using delayed addition of tracer antigen, the method is sensitive (9.5 reproducible and specific. A good parallel relationship was observed between the standard curve and dilution curves for human urine and human pancreatic juice.
Takesada Mori, Noshi Minamiura, Keisuke Miyauchi, Takashi Shibata, Kazuhiko Matsuda, Takehiko Yamamoto, Michio Ogawa, Kazuo Ito   +7 more
openaire   +3 more sources

Drosophila deoxyribonucleases. I.

Biochimica et Biophysica Acta (BBA) - Enzymology, 1969
Abstract A technique for detecting deoxyribonucleases after their fractionation by disc electrophoresis has been used to study these enzymes in Drosophila melanogaster. A complex spectrum of enzymatic activities has been found which undergoes striking variation during development. An analysis of the substrate and divalent cation requirements of these
openaire   +3 more sources

Depolymerization of F-actin by deoxyribonuclease I

Cell, 1976
Deoxyribonuclease I causes depolymerization of filamentous muscle actin to form a stable complex of 1 mole DNAase I:1 mole actin. The regulatory proteins tropomyosin and troponin bind to filamentous actin and slow down but do not prevent the depolymerization. In the absense of ATP, heavy meromyosin binds tightly to actin filaments and blocks completely
Lars Carlsson, Sarah E. Hitchcock, Uno Lindberg   +2 more
openaire   +3 more sources

Label-free colorimetric detection of deoxyribonuclease I activity based on the DNA-enhanced peroxidase-like activity of MIL-53(Fe)

New Journal of Chemistry, 2019
In this study, the effect of single-stranded DNA (ssDNA) on the intrinsic peroxidase-like activity of MIL-53(Fe) was investigated.
Chan Song, W. Ding, Haibo Liu, Weiwen Zhao, Yuewei Yao, Cheng Yao   +5 more
semanticscholar   +1 more source