Results 191 to 200 of about 14,183,395 (235)

Multiple forms of deoxyribonuclease I

Molecular and Cellular Biochemistry, 1981
This article will review recent progress on the purification of DNase I (E.C.3.1.4.5) from various sources and the characterization of multiple forms of the enzyme. The chemical basis of the multiple forms in bovine pancreas will be discussed in detail, while for other DNases, including those in ovine pancreas, bovine, mouse and rat parotid, and malt ...
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Active Site of Deoxyribonuclease I: II. The Mechanisms of the Radiation-Induced Inactivation of Deoxyribonuclease I in Aqueous Solution

Radiation Research, 1962
The kinetic properties of irradiated deoxyribonuclease in aqueous solution were determined. They show that the maximum velocity and the Michaelis- Menten constant decreased with increasing radiation dose. The D/sub 37/ doses of the irradiated enzyme assayed at the low substrate concentrations were higher than those at the high substrate concentrations.
S. Okada, G. Fletcher
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Structure and Function of Bovine Pancreatic Deoxyribonuclease I

Protein & Peptide Letters, 2006
Bovine pancreatic deoxyribonuclease I (bpDNase), the first DNase discovered, is the best characterized among various types of DNase. A catalytic mechanism has been suggested based on the X-ray structure of the bpDNase-octamer complex. In this review, we will focus on three aspects: 1) the distinctive functions of the two structural calcium atoms; 2 ...
Wei-Jung Chen, Ta-Hsiu Liao
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Characterization of Preferred Deoxyribonuclease I Cleavage Sites

Journal of Molecular Biology, 1994
The preferred DNase I cleavage sites within the 160 bp tyrT DNA fragment were identified by studying the initial rate of cleavage of individual bonds. The results show that there is no correlation between the rate of cleavage and the identity of the dinucleotide sequence that is cleaved.
Julio E. Herrera, Jonathan B. Chaires
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Fluorometric Determination of Deoxyribonuclease I Activity with PicoGreen

Analytical Biochemistry, 2000
A rapid and sensitive assay for the detection of deoxyribonuclease I (DNase I) activity is described. This method is based on the ability of PicoGreen dye to enhance its fluorescence when bound to double-stranded DNA. In the standard assay, reaction mixtures containing the DNase I sample and 0.2 microg of the substrate DNA were prepared in a ...
Francis C. Szoka, Suk-Jung Choi
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Genetic polymorphism of human urine deoxyribonuclease I

Human Genetics, 1989
A genetic polymorphism of human urine deoxyribonuclease I (DNase I) has been detected by the technique of polyacrylamide gel isoelectric focusing (IEF-PAGE) followed by immunoblotting with anti-DNase I antibody. Family studies showed that the three common phenotypes - DNASE1 1, 1-2, and 2 - and the other four rare phenotypes - DNASE1 1-3, 2-3, 2-4, and
Toshihiro Yasuda, Koichiro Kishi, Shuichi Awazu, Keiko Mizuta   +3 more
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Survey of the Association of Deoxyribonuclease I Polymorphism with Disease

Human Heredity, 1993
The deoxyribonuclease I (DNase I) system was studied in 120 unrelated Japanese patients with liver disease, malignant neoplasms, alimentary-canal disease and inflammatory conditions with respect to the distribution of phenotypes and gene frequencies in serum samples.
Etsuko Tenjo, Haruo Takeshita, Toshihiro Yasuda, Koichiro Kishi, Masao Nakanaga, Daita Nadano   +5 more
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Thiazole derivatives as dual inhibitors of deoxyribonuclease I and 5-lipoxygenase: A promising scaffold for the development of neuroprotective drugs.

Chemico-Biological Interactions, 2023
Ana Marković, A. Živković, M. Atanasova, I. Doytchinova, B. Hofmann, S. George, S. Kretschmer, Carmen B Rödl, D. Steinhilber, H. Stark, A. Šmelcerović   +10 more
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Late Deoxyribonuclease Activity ofSalmonella enteritidis

American Journal of Clinical Pathology, 1979
As a result of chance observations, the authors studied for deoxyribonuclease activity 16 strains of Salmonella, including six fresh isolates and ten stock cultures, with positive results in 13. Reactions characteristically occurred at 48 hours or later, with the majority being manifest at 72 hours and the latest at six days. No other positive reaction
Michael Tomasulo, Herbert Braunstein
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Deoxyribonuclease I Phenotyping from Saliva Stains

Journal of Forensic Sciences, 1999
Abstract Good typing results were obtained using a newly developed method for extraction and purification of deoxyribonuclease I (DNase I) from saliva stains. Previously, DNase I phenotyping from saliva stains has been unsuccessful because of low enzyme activity and heavy contamination.
Reiko Iida, M Aoyama, Toshihiro Yasuda, Koichiro Kishi, Etsuko Tsubota, Takasumi Matsuki, Kazumi Sawazaki   +6 more
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