Results 81 to 90 of about 104,722 (311)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Feasibility Study and Porting of the Damped Least Square Algorithm on FPGA

IEEE Access, 2020
Modern embedded computing platforms used within Cyber-Physical Systems (CPS) are nowadays leveraging more and more often on heterogeneous computing substrates, such as newest Field Programmable Gate Array (FPGA) devices.
Carlo Sau, Tiziana Fanni, Claudio Rubattu, Luca Fanni, Luigi Raffo, Francesca Palumbo   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Physical design automation of structured high-performance integrated circuits [PDF]

, 2014
textDuring the last forty years, advancements have pushed state-of-the-art placers to impressive performance placing modern multimillion gate designs in under an hour.
Ward, Samuel Isaac
core  

Tracking Control of A Balancing Robot – A Model-Based Approach

Archive of Mechanical Engineering, 2014
This paper presents a control concept for a single-axle mobile robot moving on the horizontal plane. A mathematical model of the nonholonomic mechanical system is derived using Hamel's equations of motion.
Zaiczek Tobias, Franke Matthias
doaj   +1 more source

Automating Benchmark Design

CoRR
The rapid progress and widespread deployment of LLMs and LLM-powered agents has outpaced our ability to evaluate them. Hand-crafted, static benchmarks are the primary tool for assessing model capabilities, but these quickly become saturated. In contrast, dynamic benchmarks evolve alongside the models they evaluate, but are expensive to create and ...
Amanda Dsouza, Harit Vishwakarma, Zhengyang Qi, Justin Bauer, Derek Pham, Thomas Walshe, Armin Parchami, Frederic Sala, Paroma Varma   +8 more
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Enhancing lower-limb rehabilitation: a scoping review of augmented reality environment

Journal of NeuroEngineering and Rehabilitation
Background Lower-limb rehabilitation is crucial for restoring motor function in individuals with physical impairments; however, traditional rehabilitation approaches often encounter challenges such as limited resources and reduced patient motivation ...
Yuanyuan Liu, Qiong Zhang, Weiyi Li
doaj   +1 more source

Evaluating the Effectiveness of Autorouting in PCB Design

Engineering Proceedings
This research aims to determine an optimized set of parameters for Altium Designer’s built-in autorouting function when applied on two- or more-layer printed circuit board (PCB) designs.
Péter Gulyás
doaj   +1 more source