Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Multigap superconductivity in the Mo5PB2 boron–phosphorus compound
New Journal of Physics, 2020 The tetragonal Mo _5 PB _2 compound was recently reported to show superconductivity with a critical temperature up to 9.2 K. In search of evidence for multiple superconducting gaps in Mo _5 PB _2 , comprehensive measurements, including magnetic ...
T Shang +9 more
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Statistical considerations in design of spacelab experiments [PDF]
After making an analysis of experimental error sources, statistical models were developed for the design and analysis of potential Space Shuttle experiments.
Robinson, J.
core +1 more source
Policy, design and management: the in-vivo laboratory for the science of complex socio-technical systems [PDF]
, 2009 Complex systems scientists cannot by themselves perform experiments on complex socio-technical systems. The best they can do is to perform experiments alongside policy makers who are constantly engaged in experiments as the design and manage the systems ...
Johnson, Jeffrey
core
Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids
Molecular Oncology, EarlyView.This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici +8 more
wiley +1 more source
Design and Analysis of Monte Carlo Experiments [PDF]
monte carlo experiments;simulation models;mathematical analysis;sensitivity analysis;experimental ...
Kleijnen, J.P.C.
core +1 more source
On Metamodeling of Electronic Circuits using Efficient Experiment Plans [PDF]
Journal of Electrical and Electronics Engineering, 2020 The use of metamodels has become more and more frequent when it comes to the analysis and optimization of simulations, as they are capable of predicting the behavior of a system in a much shorter time compared to normal simulations, but with comparable ...
CĂRBUNESCU-STOENESCU Bianca-Raluca +4 more
doaj
A synthetic benzoxazine dimer derivative targets c‐Myc to inhibit colorectal cancer progression
Molecular Oncology, EarlyView.Benzoxazine dimer derivatives bind to the bHLH‐LZ region of c‐Myc, disrupting c‐Myc/MAX complexes, which are evaluated from SAR analysis. This increases ubiquitination and reduces cellular c‐Myc. Impairing DNA repair mechanisms is shown through proteomic analysis.
Nicharat Sriratanasak +8 more
wiley +1 more source