Results 101 to 110 of about 21,433,054 (296)

Spatially and Temporally Resolved Analysis of Bleeding in a Centrifugal Partition Chromatography Rotor

open access: yesSeparations
Centrifugal Partition Chromatography (CPC) is a separation technique that utilizes immiscible liquid phases to purify compounds. The selection of solvents in Liquid–Liquid Chromatography offers flexibility and optimization possibilities for specific ...
Felix Buthmann   +4 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

open access: yesFEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee   +7 more
wiley   +1 more source

Enhanced Protein Recovery from Rapeseed Press Cake via Pressurized Liquid Extraction: Effects of pH Shifting and Process Parameters

open access: yesApplied Sciences
Rapeseed press cake (RPC), the protein-rich residue from edible oil production, is currently underutilized and is primarily used as animal feed. This study aimed to investigate pressurized liquid extraction (PLE) for protein recovery from RPC using ...
Christoforos Vasileiou   +4 more
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

open access: yesFEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar   +4 more
wiley   +1 more source

Design and analysis strategies for robust microbiome ageing research

open access: yesFEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik   +5 more
wiley   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

First-principle modeling of parallel-flow regenerative kilns and their optimization with genetic algorithm and gradient-based method

open access: yesDigital Chemical Engineering
We present a one-dimensional first-principle model for parallel-flow regenerative kilns that accounts for the most important effects. These include the kinetics and thermal effects of the limestone decomposition as well as the heat transfer between the ...
Michael Kreitmeir   +4 more
doaj   +1 more source

The Design Process Explained: Video 1 Introduction

open access: yes, 2019
This video provides an introduction to the Double Diamond Design Process and provides an overview of the whole video series, showing brief examples from each stage of the design process and why it is ...
Charlie Ranscombe, Kate Bissett-Johnson
core  

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Project Process Cards: A Self Evaluation Tool for Design Studio

open access: yes, 2019
Industrial design education is about process rather than product. Design education requires students to follow particular paths for learning design practice during their journey to proposing design solutions.
Öztoprak, Aydın   +5 more
core   +1 more source

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