Results 81 to 90 of about 390,801 (245)
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
RESUMEN: Este artículo analiza el sexto libro de la poeta canadiense Sarah Klassen, Simone Weil: Songs of Hunger and Love (1999), una colección de poemas y breves piezas en prosa donde la autora presta su primera persona a Simone Weil, filósofa francesa ...
Nieves Pascual Soler
doaj
This paper explores the impact of gender differences in the desire for sex and the distribution of power in the household on the onset of the demographic transition and the take-off to growth. Depending on the price and efficacy of modern contraceptives, the gender wage gap, and female bargaining power, the economy assumes one of two possible ...
openaire +4 more sources
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
THE CITY AND THE RESURFACING OF THE REPRESSED. SPACE IN THE NOVEL U REGISTRATURI BY ANTE KOVAČIĆ
Together with the decline of the nobility, the city schooling of a country child and the twofold village-city relationship are the key themes of Croatian realism, as well as realism world-wide.
Dejan Durić
doaj
This study underscores the significant influence of frailty and vitality on the subjective health experience of older cancer survivors with acceptance and control emerging as salient mediators. These findings affirm the conceptual and empirical robustness of the model highlighting its potential utility in shaping future interventions for older cancer ...
Damien S. E. Broekharst +4 more
wiley +1 more source
Revisión de la teología agustiniana con una original teoría sobre el deseo. (Primera parte) / Reviewing Augustinian Theology by an original theory of desire. (Part 1)) [PDF]
La tradición cristiana se fundamenta sobre tres pilares: el pecado original, el Mediador, y el sacrificio, que sustentan una filosofía de la historia. San Agustín es uno de los exponentes de este esquema, pero necesita un lenguaje actualizado, frente a ...
Ángel Jorge Barahona Plaza
doaj

