Results 151 to 160 of about 913,709 (314)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Adaptive Dynamic Thresholding Method for Fault Detection in Diesel Engine Lubrication Systems

Machines
Fault detection in marine diesel engine lubrication systems is crucial for ensuring the long-term stable operation of diesel engines and the safety of maritime navigation. Traditional fixed-parameter alarm threshold methods lack flexibility and are prone
Tingting Wu, Hongliang Song, Hongli Gao, Zongshen Wu, Feifei Han   +4 more
doaj   +1 more source

Taste detection threshold of human (Homo sapiens) subjects and taste preference threshold of black-handed spider monkeys (Ateles geoffroyi) for the sugar substitute isomalt. [PDF]

Primates, 2021
Pereira S, Hernandez Salazar LT, Laska M.   +2 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Enhanced federated anomaly detection through autoencoders using summary statistics-based thresholding

Scientific Reports
In Federated Learning, Anomaly Detection poses significant challenges due to the decentralized nature of data, especially under Non-IID distributions. This study proposes a federated threshold calculation method that aggregates summary statistics from ...
Sofiane Laridi, Gregory Palmer, Kam-Ming Mark Tam   +2 more
doaj   +1 more source

Research on distributed cooperative algorithm based on signal-to-noise ratio coefficient with double thresholds

Dianzi Jishu Yingyong
In order to solve the problem that the traditional energy detection algorithm has poor detection performance due to the fixed decision threshold when the signal-to-noise ratio is low, this paper studies a dual-threshold cooperative energy detection ...
Li Zhanxiong, Liu Liping, Fang Liang, Lv Jiafeng, Wang Ci   +4 more
doaj   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source