Results 1 to 10 of about 273,322 (239)

Explicit Kinetic Heterogeneity: Mathematical Models for Interpretation of Deuterium Labeling of Heterogeneous Cell Populations [PDF]

PLoS Computational Biology, 2010
Estimation of division and death rates of lymphocytes in different conditions is vital for quantitative understanding of the immune system. Deuterium, in the form of deuterated glucose or heavy water, can be used to measure rates of proliferation and ...
Vitaly Ganusov, Rob J. De Boer, José Borghans   +2 more
exaly   +4 more sources

Rapid Antibiotic Susceptibility Testing by Deuterium Labeling of Bacterial Lipids in On-Target Microdroplet Cultures [PDF]

Journal of the American Society for Mass Spectrometry, 2022
Antimicrobial resistance is a serious challenge facing human and veterinary health. Current methods of detecting resistance are limited in turn-around time or universal detection.
Young Jin Lee, Bryan H Bellaire, Kristina Larsen   +2 more
exaly   +4 more sources

Stable Deuterium Labeling of Histidine-Rich Lysine-Based Dendrimers [PDF]

Molecules, 2019
Peptide dendrimers, due to their biocompatibility and low toxicity, are highly promising candidates as nanocarriers for drugs and genes. The development of this kind of delivery system requires reliable monitoring of their metabolic and biological ...
Nadezhda N. Sheveleva, Denis A. Markelov, Mikhail A. Vovk, Irina I. Tarasenko, Mariya E. Mikhailova, Maxim Yu Ilyash, Igor M. Neelov, Erkki Lahderanta   +7 more
doaj   +2 more sources

Deuterium labeling enables proteome-wide turnover kinetics analysis in cell culture [PDF]

Cell Reports: Methods
Summary: Protein turnover is a critical component of gene expression regulation and cellular homeostasis, yet methods for measuring turnover rates that are scalable and applicable to different models are still needed.
Lorena Alamillo, Dominic C.M. Ng, Jordan Currie, Alexander Black, Boomathi Pandi, Vyshnavi Manda, Jay Pavelka, Peyton Schaal, Joshua G. Travers, Timothy A. McKinsey, Maggie P.Y. Lam, Edward Lau   +11 more
doaj   +2 more sources

Heavy water labeling reveals metabolic flexibility of amino acid and polyamine pathways in mammalian cells [PDF]

Communications Chemistry
Amino acid and polyamine metabolism underpins many cellular processes, such as cell growth, stress adaptation, and signaling. However, the usage of specific metabolic pathways is highly context-dependent, and there are many compensatory mechanisms in ...
Alan Gonzalez-Ibarra, Missael Arroyo-Negrete, Wioleta Banaszuk-Krupa, Katarzyna Socała, Nikola Gapińska, Piotr Wlaź, Julio Cesar Torres-Elguera, Tomasz Sawoszczuk, Marek Tchórzewski, Maria Hatzoglou, Dawid Krokowski   +10 more
doaj   +2 more sources

2H2O labeling methods for bulk and single muscle protein synthesis measures, and measures of integrated muscle protein synthesis and breakdown rates: A pilot study [PDF]

Physiological Reports
This methodological pilot study explored the feasibility of an integrated tracer approach for simultaneous measurement of human skeletal muscle protein synthesis and breakdown during prolonged deuterium oxide (2H2O) labeling. Four healthy men completed a
Grith Højfeldt, Bjørk Wulff Helge, Gerrit vanHall, Peter Schjerling, Michael Kjær, Abigail L. Mackey, Jatin G. Burniston, Jakob Agergaard   +7 more
doaj   +2 more sources

Protocol to measure protein half-life in cell culture using heavy water [PDF]

STAR Protocols
Summary: Protein turnover, comprising the continued synthesis and clearance of proteins, is required for protein homeostasis and cell survival. Here, we present a protocol to measure protein turnover half-life in cultured cells using D2O labeling.
Lorena Alamillo, Alexander Black, Matthew C. Juber, Dominic C.M. Ng, Maggie P.Y. Lam, Edward Lau   +5 more
doaj   +2 more sources

In vivo deuterium magnetic resonance imaging of xenografted tumors following systemic administration of deuterated water

Scientific Reports, 2023
In vivo deuterated water (2H2O) labeling leads to deuterium (2H) incorporation into biomolecules of proliferating cells and provides the basis for its use in cell kinetics research.
Jeffrey R. Brender, Julian C. Assmann, Don E. Farthing, Keita Saito, Shun Kishimoto, Kathrynne A. Warrick, Natella Maglakelidze, Terri L. Larus, Hellmut Merkle, Ronald E. Gress, Murali C. Krishna, Nataliya P. Buxbaum   +11 more
doaj   +1 more source

An In Vivo Stable Isotope Labeling Method to Investigate Individual Matrix Protein Synthesis, Ribosomal Biogenesis, and Cellular Proliferation in Murine Articular Cartilage

Function, 2022
Targeting chondrocyte dynamics is a strategy for slowing osteoarthritis progression during aging. We describe a stable-isotope method using in vivo deuterium oxide labeling and mass spectrometry to measure protein concentration, protein half-life, cell ...
Kamil A Kobak, Albert Batushansky, Agnieszka K Borowik, Erika Prado Barboza Lopes, Frederick F Peelor, Elise L Donovan, Michael T Kinter, Benjamin F Miller, Timothy M Griffin   +8 more
doaj   +1 more source

Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy

Plant Methods, 2023
Background Investigations into the growth and self-organization of plant roots is subject to fundamental and applied research in various areas such as botany, agriculture, and soil science.
Yalda Davoudpour, Steffen Kümmel, Niculina Musat, Hans Hermann Richnow, Matthias Schmidt   +4 more
doaj   +1 more source