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In Silico Analysis of the Dextransucrase Obtained From Strain IBUN 91.2.98 [PDF]

open access: yesBioinformatics and Biology Insights, 2023
The DSR-IBUN dextransucrase produced by Leuconostoc mesenteroides strain IBUN 91.2.98 has a short production time (4.5 hours), an enzymatic activity of 24.8 U/mL, and a specific activity of purified enzyme 2 times higher (331.6 U/mg) than that reported ...
Luisa Alejandra García Galindo   +3 more
doaj   +2 more sources

Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols [PDF]

open access: yesBiochemistry and Biophysics Reports, 2021
Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans.
Dimple Goyal   +3 more
doaj   +2 more sources

The C-Terminal Domain of Liquorilactobacillus nagelii Dextransucrase Mediates the Production of Larger Dextrans Compared to Liquorilactobacillus hordei [PDF]

open access: yesGels, 2022
Dextransucrases released by certain lactic acid bacteria form glucose polymers with predominantly α-1,6-linkages and may be exploited biotechnologically for the tailored production of polysaccharides with application potential.
Julia Bechtner   +4 more
doaj   +2 more sources

Low Molar Mass Dextran: One-Step Synthesis With Dextransucrase by Site-Directed Mutagenesis and its Potential of Iron-Loading [PDF]

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Iron dextran is a common anti-anemia drug, and it requires low molar mass dextran as substrate. In this work, we selected 11 amino acid residues in domain A/B of DSR-MΔ2 within a 5-angstrom distance from sucrose for site-directed mutagenesis by molecular
Tingting Wang   +8 more
doaj   +2 more sources

Combinatorial engineering of dextransucrase specificity. [PDF]

open access: yesPLoS ONE, 2013
We used combinatorial engineering to investigate the relationships between structure and linkage specificity of the dextransucrase DSR-S from Leuconostoc mesenteroides NRRL B-512F, and to generate variants with altered specificity.
Romain Irague   +7 more
doaj   +7 more sources

One-step enzymatic synthesis of medium molecular weight dextran using engineered dextransucrase DarM from Leuconostoc citreum CBA3623 [PDF]

open access: yesFrontiers in Microbiology
The dextransucrase DarM from Leuconostoc citreum CBA3623, a large GH70 family enzyme of approximately 213 kDa, was refractory to full-length expression in E. coli.
Wenchao Zhang   +11 more
doaj   +2 more sources

The Discovery, Molecular Cloning, and Characterization of Dextransucrase LmDexA and Its Active Truncated Mutant from Leuconostoc mesenteroides NN710 [PDF]

open access: yesMolecules
Dextransucrases play a crucial role in the production of dextran from economical sucrose; therefore, there is a pressing demand to explore novel dextransucrases with better performance.
Xiaoqiong Zuo   +6 more
doaj   +2 more sources

Brewers’ spent grain as substrate for dextran biosynthesis by Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16 [PDF]

open access: yesMicrobial Cell Factories, 2021
Background Lactic acid bacteria can synthesize dextran and oligosaccharides with different functionality, depending on the strain and fermentation conditions.
Prabin Koirala   +4 more
doaj   +2 more sources

Living the Sweet Life: How Liquorilactobacillus hordei TMW 1.1822 Changes Its Behavior in the Presence of Sucrose in Comparison to Glucose [PDF]

open access: yesFoods, 2020
Liquorilactobacillus (L.) hordei (formerly Lactobacillus hordei) is one of the dominating lactic acid bacteria within the water kefir consortium, being highly adapted to survive in this environment, while producing high molecular weight dextrans from ...
Julia Bechtner   +4 more
doaj   +2 more sources

Safety evaluation of the food enzyme dextransucrase from the genetically modified Bacillus subtilis strain DP‐Eyp97 [PDF]

open access: yesEFSA Journal
The food enzyme dextransucrase (sucrose:1,6‐α‐d‐glucan 6‐α‐d‐glucosyltransferase; EC 2.4.1.5) is produced with the genetically modified Bacillus subtilis strain DP‐Eyp97 by Genencor International B.V.
EFSA Panel on Food Enzymes (FEZ)   +18 more
doaj   +2 more sources

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