Results 41 to 50 of about 728 (155)
A comprehensive genomic and phenotypic analysis of Limosilactobacillus fermentum CRL2085 revealed key probiotic and nutritional traits supporting its safe application in cattle feed. The strain harbors genes for stress tolerance, adhesion, exopolysaccharide production, and B‐vitamin biosynthesis, and efficiently metabolizes plant‐derived carbohydrates,
Cecilia M. Aristimuño Ficoseco +9 more
wiley +1 more source
The extracellular dextransucrase produced from Pediococcus pentosaceus, a new isolate from the soil in Assam, India, was purified and characterized. The enzyme activity of cell-free supernatant was 3.4 U/mL and specific activity was 0.6 U/mg.
Damini Kothari, Seema Patel, Arun Goyal
doaj
Recombinant Expression of Dextransucrase in Food-grade Lactic Acid Bacteria
In order to study the synthesis of dextran in Lactobacillus and to analyze its structure, in this study, the dextransucrase DsrB was ligated with the Lactococcus lactis expression vector pNZ8148 plasmid vector with SP45 secretion signal peptide, and ...
Yuanhao PU +5 more
doaj +1 more source
Functional role and folding properties of the glucan‐binding domain of oral bacterial glucansucrase
The role of the glucan‐binding domain in Streptococcus sobrinus glucansucrase was examined, focusing on its impact on enzymatic activity, dextran binding, and structural stability of deletion mutants and a circularly permuted protein. Our research revealed that glucosyl transfer efficiency is linked to cooperative interdomain folding.
Hideyuki Komatsu +5 more
wiley +1 more source
Abstract The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022.
David J. Harvey
wiley +1 more source
Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom.
Michelena Georgina L. +4 more
doaj
Background Dextransucrases are extracellular enzymes, which catalyze the formation of α-1→6-linked glucose polymers from sucrose. These enzymes are exclusively expressed by lactic acid bacteria, which commonly acidify the extracellular environment due to
Jonas Schmid +3 more
doaj +1 more source
Abstract The qualified presumption of safety (QPS) process was developed to provide a safety assessment approach for microorganisms intended for use in food or feed chains. In the period covered by this Statement, no new information was found that would change the status of previously recommended QPS taxonomic units (TUs).
EFSA Panel on Biological Hazards (BIOHAZ) +28 more
wiley +1 more source
The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type.
Seema Patel +4 more
doaj +1 more source
Hydroxyapatite: An Eco‐Friendly Material for Enzyme Immobilization
This minireview provides a literature survey on the use of hydroxyapatite (HAP) as a sustainable and ecofriendly support for enzyme immobilization, a key technology for the design of stable, efficient and reusable biocatalysts. The different immobilization techniques used for obtaining biocatalysts based on HAP and HAP‐composites are considered and ...
Leonardo Gelati +6 more
wiley +1 more source

