Bottom‐Up Synthesized Glucan Materials: Opportunities from Applied Biocatalysis
Linear d‐glucans, simple yet versatile polysaccharides, shape nanostructures crucial for biomaterial development. Shifting from top‐down to bottom‐up synthesis using natural and engineered enzymes provides meticulous control over polymer structures, fostering diversification for macromolecular materials.
Chao Zhong, Bernd Nidetzky
wiley +1 more source
Levansucrases from Gluconobacter oxydans, Vibrio natriegens, Novosphingobium aromaticivorans, and Paraburkholderia graminis were used to catalyze the transfructosylation of phenolic compounds and carbohydrates. The selected phenolic acceptor substrates were successfully glycosylated with up to five fructosyl groups transferred from the sucrose donor ...
Muriel Wong Min, Lan Liu, Salwa Karboune
wiley +1 more source
Rapid Filter Paper Assay for the Dextransucrase Activity from Streptococcus mutans
A convenient, sensitive, and reliable assay for the conversion of radiolabeled sucrose to alcohol-insoluble dextran by the Streptococcus mutans dextransucrase has been developed.
Andrew M. Chludzinski +2 more
core +1 more source
Specific method for the purification of Streptococcus mutans dextransucrase [PDF]
A convenient and rapid method for the purification of Streptococcus mutans dextransucrase is described. Affinity chromatography, on a column containing insoluble dextran purified from a culture of S. mutans 6715-49, gave an almost 300-fold purification, with 76% recovery of enzyme.
M M, McCabe, E E, Smith
openaire +2 more sources
Effect of addition of wheat bran hydrolysate on bread properties
Abstract Although the addition of bran to bread makes it healthier and more functional, it brings with it some technological problems. One way to eliminate these problems is hydrothermal pretreatment of wheat bran. In this study, five different ratios (10%, 20%, 30%, 50%, and 100%) of hydrolysates from hydrothermal pretreatment of wheat bran (150°C, 30
Ali Cingöz +2 more
wiley +1 more source
Oxidative / Nitrosative Stress ss in Chronic Heart Failure: A Critical Review [PDF]
Heart failure constitutes a major cardiovascular health hazard worldwide. It afflicts over 1% of the general population and nearly 1 in 10 of the elderly.
Doğan Yücel +3 more
doaj
RETRACTED ARTICLE: Screening and Optimization of Dextransucrase Production from Lactobacillus acidophilus [PDF]
Background: Dextransucrase is an enzyme synthesized by many bacterial genera, which catalyzes the hydrolysis of sucrose to produce dextran, a polysaccharide with a wide range of medical, pharmaceutical, and industrial applications.
Muslim, Sahira Nsayef +2 more
core +1 more source
Purification, surfactant stabilization, molecular weight studies, and divalent metal ion kinetics of dextransucrase from Leuconostoc mesenteroides NRRL B-512F [PDF]
Dextransucrase from Leuconostoc mesenteroides NRRL B-512F, an enzyme that synthesizes an (alpha)-(1(--->)6)-linked glucan with (alpha)-(1(--->)3)-linked branches from the glucosyl moiety of sucrose, was stabilized against thermal inactivation and against
Miller, Arthur
core +1 more source
Chemical synthesis of sucrose analogues and the study of their inhibition of dextransucrase [PDF]
Several analogues of sucrose were synthesized and tested as inhibitors of dextransucrase. These compounds were as follows: xylsucrose, (alpha)-D-xylopyranosyl fluoride, 6,6'-dideoxy-6,6'-dibromosucrose, 6,6'-dideoxy-6,6'-dichlorosucrose, 6,6'-dideoxy-6,6'
Zikopoulos, John
core +1 more source
Studies on Dextransucrase Part VI
(1) ショ糖培地に生育させたLeuc. mesenteroidesの菌体を音波処理して調製した無緬胞抽出液を,B2グルコシドに作用するとB2を遊離した.また抽出液をB2イソマルトシドに作用すると,B2イソマルトシドはB2グルコシドを経てB2へ,またB2イソマルトトリオシドに作用すると,B2イソマルトトリオシドはB2イソマルトシド,B2グルコシドを経てB2へ順次分解された.B2にグルコース分子が平均10個,あるいは18個結合したようなB2高級少糖類も徐々に,それぞれグルコース分子の結合数がより少ないB2少糖類へ分解された. (2)硫酸アンモニウム分別塩析法によってB2グルコシル化合物の生成および分解の両活性区分が大別された.
SUZUKI, Yukio, UCHIDA, Kei
openaire +3 more sources

