Structure of a eukaryotic cholinephosphotransferase-1 reveals mechanisms of substrate recognition and catalysis [PDF]
Nature Communications, 2023 Phosphatidylcholine (PC) is the most abundant phospholipid in eukaryotic cell membranes. In eukaryotes, two highly homologous enzymes, cholinephosphotransferase-1 (CHPT1) and choline/ethanolamine phosphotransferase-1 (CEPT1) catalyze the final step of de
Lie Wang, Ming Zhou
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The seed‐specific transcription factor DPBF2 modulates the fatty acid composition in seeds
Plant Direct, 2022 Triacylglycerol (TAG), an ester derived from glycerol and three fatty acids (FAs), is synthesized during seed development and controlled by transcriptional regulation.
Inyoung Kim +3 more
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Enhanced production of hydroxy fatty acids in Arabidopsis seed through modification of multiple gene expression [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Background Castor (Ricinus communis L.) seeds contain unusual fatty acid, hydroxy fatty acid (HFA) used as a chemical feedstock for numerous industrial products.
Mid-Eum Park +3 more
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Frontiers in Plant Science, 2020 Camelina sativa is an emerging biotechnology oil crop. However, more information is needed regarding its innate lipid enzyme specificities. We have therefore characterized several triacylglycerol (TAG) producing enzymes by measuring in vitro substrate ...
Ida Lager +7 more
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Solubilization and Purification of Rat, Liver Microsomal 1,2‐Diacylglycerol: CDP‐choline Cholinephosphotransferase and 1,2‐Diacylglycerol CDP‐ethanolamine Ethanolaminephosphotransferase [PDF]
European Journal of Biochemistry, 1976 The procedure, which involved 2‐step sonication of microsomes at pH 7.4 and then at pH 8.5 in the presence of sodium deoxycholate and subsequent dialysis, resulted in 4–5‐fold purification of choline‐phosphotransferase and ethanolaminephosphotransferase with the yield of 40–50%.
H, Kanoh, K, Ohno
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Overproduction of Phospholipids by the Kennedy Pathway Leads to Hypervirulence in Candida albicans
Frontiers in Microbiology, 2019 Candida albicans is an opportunistic human fungal pathogen that causes life-threatening systemic infections, as well as oral mucosal infections. Phospholipids are crucial for pathogenesis in C.
Robert N. Tams +7 more
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The Phosphatidylcholine Diacylglycerol Cholinephosphotransferase Is Required for Efficient Hydroxy Fatty Acid Accumulation in Transgenic Arabidopsis [PDF]
Plant Physiology, 2012 AbstractWe previously identified an enzyme, phosphatidylcholine diacylglycerol cholinephosphotransferase (PDCT), that plays an important role in directing fatty acyl fluxes during triacylglycerol (TAG) biosynthesis. The PDCT mediates a symmetrical interconversion between phosphatidylcholine (PC) and diacylglycerol (DAG), thus enriching PC-modified ...
Zhaohui, Hu, Zhonghai, Ren, Chaofu, Lu
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Cholinephosphotransferase and Diacylglycerol Acyltransferase (Substrate Specificities at a Key Branch Point in Seed Lipid Metabolism) [PDF]
Plant Physiology, 1996 Many oilseed plants accumulate triacylglycerols that contain unusual fatty acyl structures rather than the common 16- and 18-carbon fatty acids found in membrane lipids of these plants. In vitro experiments demonstrate that triacylglycerols are synthesized via diacylglycerols in microsomal preparations and that this same sub-cellular fraction is the ...
G., Vogel, J., Browse
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Kinetic selectivity of cholinephosphotransferase in mouse liver: the Km for CDP-choline depends on diacylglycerol structure [PDF]
Biochemical Journal, 1993 The effects of different 1,2-diacyl-sn-glycerols on the kinetic properties of CDP-choline:1,2-diacylglycerol cholinephosphotransferase (EC 2.7.8.2) from mouse liver microsomes have been studied. Initial-velocity experiments were carried out with various concentrations of several species of diacylglycerol at different fixed concentrations of CDP-choline.
C R, Mantel +3 more
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Journal of Biological Chemistry, 1977 Diacylglycerol cholinephosphotransferase (EC 2.7.8.2) and diacylglycerol ethanolaminephosphotransferase (EC 2.7.8.1) activities were investigated in microsomes from isolated rat fat cells. Assays based on the conversion of CDP-[14C]choline of CDP-[14C]ethanolamine to phosphatidylcholine or phosphatidylethanolamine utilized ethanol-dispersed ...
R, Coleman, R M, Bell
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