A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development [PDF]
We describe the application of a novel image processing method, which allows quantitative analysis of cell and tissue movement in a series of digitized video images. The result is a vector velocity field showing average direction and velocity of movement
Miike, Hidetoshi+3 more
core +1 more source
Dictyostelium discoideum Electroporator
Easily constructed by investigators with no electronics knowledge, we present an inexpensive and open-source electroporation apparatus for Dictyostelium discoideum with all functional modules and peripherals from Cauchy et al., 2022, "A Low-cost Electroporator for Genetically Modifying Social Amoeba Dictyostelium discoideum" submitted to Journal of ...
Michael C. Cauchy+3 more
openaire +2 more sources
Fate and regulation of anterior-like cells in Dictyostelium slugs [PDF]
A pattern of two tissue types exists in the Dictyostelium slug. Contained within the posterior tissue are anterior-like cells which comprise about 10% of the developing cell mass.
David, Charles N., Sternfeld, John
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Digital image processing of optical density wave propagation in Dictyostelium discoideum and analysis of the effects of caffeine and ammonia [PDF]
Waves of chemotactic movement during the early phase of aggregation in Dictyostelium discoideum were analyzed by digital image processing in a manner that immediately shows the following parameters: wave propagation velocity, period length, wave ...
Siegert, Florian, Weijer, Cornelis J.
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Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum
Development of the soil amoeba Dictyostelium discoideum is triggered by starvation. When placed on a solid substrate, the starving solitary amoebae cease growth, communicate via extracellular cAMP, aggregate by tens of thousands and develop into ...
Rafael D. Rosengarten+6 more
semanticscholar +1 more source
Dictyostelium discoideum as a Model for Investigating Neurodegenerative Diseases
The social amoeba Dictyostelium discoideum is a model organism that is used to investigate many cellular processes including chemotaxis, cell motility, cell differentiation, and human disease pathogenesis.
Holly N. Haver+3 more
doaj +1 more source
The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA [PDF]
SummaryThe amoeba Dictyostelium discoideum can support replication of Legionella pneumophila. Here we identify the dupA gene, encoding a putative tyrosine kinase/dual-specificity phosphatase, in a screen for D.
Bloomfield, Gareth+6 more
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Mitochondrial potassium channels in Dictyostelium discoideum [PDF]
Mitochondria are crucial not only in energy metabolism but also in regulation of cell senescence and apoptosis. The strict control of inner mitochondrial membrane permeability and selective ion transport is essential for mitochondria functioning. Potassium ions homeostasis is an important process for mitochondrial optimal functioning.
Michal Laskowski+3 more
openaire +3 more sources
Cell Sorting daring Pattern Formation in Dictyostelium [PDF]
Formation of the prestalk-prespore pattern in Dictyostelium was investigated in slugs and submerged clumps of cells. Prestalk and prespore cells were identified by staining with vital dyes, which are shown to be stable cell markers.
David, Charles N., Sternfeld, John
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Development of a Positive Selection High Throughput Genetic Screen in Dictyostelium discoideum
The cellular slime mold Dictyostelium discoideum is a powerful model organism that can be utilized to investigate human health and disease. One particular strength of Dictyostelium is that it can be utilized for high throughput genetic screens.
Felicia N. Williams+4 more
doaj +1 more source