Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li +9 more
wiley +1 more source
Dietary diversity and its associations with sleep quality and chronotype in young and middle-aged adults. [PDF]
Zhao A, Chen Y, Li Z, Fan Q, Wu J.
europepmc +1 more source
Comparative antennal single‐nucleus transcriptomics reveals cell‐type‐dependent transcriptomic divergence in Bactrocera dorsalis, with similar structural cells but divergent sensory neurons. This neuronal diversification is associated with a distinct ammonia‐sensing pathway, linking female attraction to bird‐dropping‐associated cues with nutritional ...
Wei Liu +7 more
wiley +1 more source
Grazing Is Associated With Dietary Diversity and Gastrointestinal Microbiota in Subterranean Rodents. [PDF]
Ren S +8 more
europepmc +1 more source
A novel dual‐organelle proximity labeling platform, DuO‐SCOUT, decodes the complex landscape of systemic organ‐organ communication by capturing both classical and unconventional secretomes. Application in metabolic models maps the adipose‐to‐brain secretory relay, identifying selective extra‐hypothalamic sites for adipose‐derived factors and expanding ...
Fenglian Yang +7 more
wiley +1 more source
Similarities in Brazilian dietary diversity: a literature review of ethnobotanical studies on regional, vegetation, and cultural contexts. [PDF]
Dos Santos LVB +4 more
europepmc +1 more source
Herbivory‐Induced Stomatal Opening Triggers the Dispersal of an Invasive Insect Herbivore
The invasive leafminer Phthorimaea absoluta uniquely manipulates plant defenses by inducing stomatal opening in tomato. This response, mediated by the stomatal regulator IQM1, enhances the release of the repellent volatile benzaldehyde. This volatile triggers the dispersal of adult moths, facilitates colonization of new hosts, and leads to rapid ...
Limin Chen +10 more
wiley +1 more source
Dietary diversity of adolescent girls living in an urban resource-poor setting of Dhaka city: results from Nutri-CAP study. [PDF]
Farzana FD +9 more
europepmc +1 more source
Engineering CAR‐Macrophages With Advanced Delivery Systems for Tissue Repair
This review highlights how engineered macrophages equipped with chimeric antigen receptors (CAR) guide tissue repair by recognizing disease‐related targets, clearing harmful cells, and reshaping local immune environments. It summarizes macrophage biology, CAR design, delivery platforms, and functionalization strategies, and discusses emerging ...
Yixin Zhang +8 more
wiley +1 more source
Dietary diversity among reproductive aged women attending urban and rural healthcare facilities, Middle Delta, Egypt. [PDF]
Hassan NM +3 more
europepmc +1 more source

