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Molecular Biotechnology, 1998
Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as ...
J S, Zhang +3 more
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Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as ...
J S, Zhang +3 more
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2005
Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material.
Mikhail V, Matz, Ella A, Meleshkevitch
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Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material.
Mikhail V, Matz, Ella A, Meleshkevitch
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Molecular Biology Reports, 2006
Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats.
Rashmi U, Pathak, M S, Kanungo
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Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats.
Rashmi U, Pathak, M S, Kanungo
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Recent advances in differential display
Current Opinion in Immunology, 1995Differential display and RNA arbitrary primed polmerase chain reaction are methods recently designed to identify and isolate differentially expressed genes. Methodological modifications have since been introduced to streamline the techniques. The major effort has centered on how to eliminate false positives as approached from a variety of angles ...
P, Liang, A B, Pardee
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Using differential display in the study of adipocyte differentiation
Biochemical Society Transactions, 1994Study of the programme of adipose conversion displayed by mouse 3T3L1 adipocyte precursors and control 3T3C2 cells [l-31 using the recently developed differential display [4-61 technique have been initiated. The two 3T3 fibroblast cell sub-lines differ in their ability to develop the biochemical characteristics of mature adipocytes and the process can ...
W D, Edwards, H L, Jessop, A, Cryer
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Differential display and protein quantification
2007High-throughput quantitation of proteins is of essential importance for all systems biology approaches and provides complementary information on steady-state gene expression and perturbation-induced systems responses. This information is necessary because it is, e.g., difficult to predict protein concentrations from the level of mRNAs, since regulatory
Erich, Brunner +3 more
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2000
Abstract When the technique of differential display was first described in the early 1990s, sequence information existed for a few thousand human genes and some functional information was available for most of those. At about that time, the development of high throughput sequencing technologies and large genome databases, including ...
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Abstract When the technique of differential display was first described in the early 1990s, sequence information existed for a few thousand human genes and some functional information was available for most of those. At about that time, the development of high throughput sequencing technologies and large genome databases, including ...
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1997
Most biological processes rely on changes in the level and pattern of gene expression. Their molecular and functional dissection often requires the isolation and subsequent characterization of genes that are differentially expressed in space (different tissues or organs), in time (different developmental stages), or in only one sex.
George Dimopoulos, Christos Louis
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Most biological processes rely on changes in the level and pattern of gene expression. Their molecular and functional dissection often requires the isolation and subsequent characterization of genes that are differentially expressed in space (different tissues or organs), in time (different developmental stages), or in only one sex.
George Dimopoulos, Christos Louis
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Differential display as an approach to study differentiation and differentiation therapy in AML
Hematological Oncology, 2000Different subgroups of acute myeloid leukemia (AML) can be defined by the specific non-random chromosomal translocation that is present within the abnormal cell types. In one type of AML, acute promyelocytic leukemia (APL), the block in the normal process of differentiation can be circumvented by the addition of a chemical inducer, in this case ...
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2015
Die „Differential-Display“ PCR (DD-PCR) ist eine Methode, die im Jahre 1992 von Liang und Pardee eingefuhrt wurde. Es handelt sich um eine Variante der RT-PCR, da aus einer mRNA PCR-Amplifikate erzeugt werden (Zhang et al. 1998). Im Gegensatz zur konventionellen RT-PCR liegen allerdings keinerlei Informationen uber die Amplifikate vor. Mit Hilfe dieser
Hans-Joachim Müller +1 more
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Die „Differential-Display“ PCR (DD-PCR) ist eine Methode, die im Jahre 1992 von Liang und Pardee eingefuhrt wurde. Es handelt sich um eine Variante der RT-PCR, da aus einer mRNA PCR-Amplifikate erzeugt werden (Zhang et al. 1998). Im Gegensatz zur konventionellen RT-PCR liegen allerdings keinerlei Informationen uber die Amplifikate vor. Mit Hilfe dieser
Hans-Joachim Müller +1 more
openaire +1 more source