Results 41 to 50 of about 2,937,719 (310)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Diffusion bonding of gold [PDF]

Gold Bulletin, 1998
Gold has a low modulus and does not form an oxide skin when heated in the air. Added to this is its rapid self-diffusion characteristic and these three properties together make this metal suitable for joining gold to gold. This paper presents a summary of a practical diffusion-bonding method for making such joints.
Humpston, Giles, Baker, Sara J
openaire   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

Effects of diffusion bonding temperature and holding time on the microstructure and strength of diffusion bonded B4C-HfB2-SiC composite ceramic joint

Journal of Materials Research and Technology
Diffusion bonding of B4C-HfB2-SiC composite ceramic with Ti and NiCoCr medium-entropy alloy as the composite interlayer material was studied in this work. The effects of diffusion bonding temperature and holding time on the interfacial microstructure and
Langxiang Zhong, Biao Liang, Chengmin Chen, Kuijing Song, Zhihong Zhong   +4 more
doaj   +1 more source

Microstructure and mechanical properties of diffusion bonded joints of high-entropy alloy Al5(HfNbTiZr)95 and TC4 titanium alloy

Journal of Materials Research and Technology, 2021
TC4 (Ti–6Al–4V) titanium alloy and Al5(HfNbTiZr)95 refractory high-entropy alloy were successfully diffusion bonded at a temperature range of 800–1000 °C for 60 min under 5 MPa.
Yu Peng, Jinglong Li, Junmiao Shi, Shiwei Li, Jiangtao Xiong   +4 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Low-Temperature Diffusion of Au and Ag Nanolayers for Cu Bonding

Applied Sciences, 2023
With the recent rapid development of IT technology, the demand for multifunctional semiconductor devices capable of high performance has increased rapidly, and the miniaturization of such devices has also faced limitations. To overcome these limitations,
Sangmin Lee, Sangwoo Park, Sarah Eunkyung Kim   +2 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source